KD-Validated Anti-Cell Division Cycle 123 Rabbit Monoclonal Antibody - 产品中心

KD-Validated Anti-Cell Division Cycle 123 Rabbit Monoclonal Antibody #63261

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Flow cytometric analysis of CDC123 expression in HAP-1 cells using anti-CDC123 antibody (Cat#63261, 1:2,000). Green, isotype control; red,CDC123.

Immunocytochemical staining of HAP1 cells with anti-CDC123 antibody (Cat#63261, 1:1,000) . Nuclei were stained blue with DAPI;CDC123 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-CDC123 antibody (Cat#63261). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CDC123 antibody (Cat#63261, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis using anti-CDC123 antibody (Cat#63261). CDC123 expression in wild-type (WT) and CDC123 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-CDC123 antibody (Cat#63261, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

CDC123
货号：

63261
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information

Product Name	KD-Validated Anti-Cell Division Cycle 123 Rabbit Monoclonal Antibody
Aliases	CDC123; Cell Division Cycle 123; D123; Cell Division Cycle Protein 123 Homolog; C10orf7; HT-1080; PZ32; Cell Division Cycle 123 Homolog (S. Cerevisiae); Chromosome 10 Open Reading Frame 7; Cell Division Cycle 123 Homolog; Protein D123; C10ORF7
Background	Gene Name: CDC123 NCBI Gene Entry: 8872 UniProt Entry: O75794
Application Information	Molecular Weight: Predicted, 39 kDa, observed, 39 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB4760 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human CDC123
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of CDC123 expression in HAP-1 cells using anti-CDC123 antibody (Cat#63261, 1:2,000). Green, isotype control; red,CDC123.
Immunocytochemical staining of HAP1 cells with anti-CDC123 antibody (Cat#63261, 1:1,000) . Nuclei were stained blue with DAPI;CDC123 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-CDC123 antibody (Cat#63261). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CDC123 antibody (Cat#63261, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-CDC123 antibody (Cat#63261). CDC123 expression in wild-type (WT) and CDC123 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-CDC123 antibody (Cat#63261, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

CDC123
货号：

63261
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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