Anti-Semaphorin 4D Rabbit Monoclonal Antibody - 产品中心

Anti-Semaphorin 4D Rabbit Monoclonal Antibody #2500

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Flow cytometric analysis of Semaphorin 4D expression in HeLa cells using anti-Semaphorin 4D antibody (Cat#2500, 1:2,000). Green, isotype control; red, Semaphorin 4D.

Immunocytochemical staining of HeLa cells with anti-Semaphorin 4D antibody (Cat#2500, 1:1,000). Nuclei were stained blue with DAPI; Semaphorin 4D was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-Semaphorin 4D antibody (Cat#2500). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Semaphorin 4D antibody (Cat#2500, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

SEMA4D
货号：

2500
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-Semaphorin 4D Rabbit Monoclonal Antibody
Aliases	Semaphorin 4D; CD100; C9orf164; Coll-4; SEMAJ; Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) And Short Cytoplasmic Domain, (Semaphorin) 4D; Semaphorin-4D; FLJ39737; BB18; GR3; A8; Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) And Short Cytoplasmic Domain, 4D; Chromosome 9 Open Reading Frame 164; CD100 Antigen; M-Sema G; M-Sema-G; COLL4
Background	Gene Name: SEMA4D NCBI Gene Entry: 10507 UniProt Entry: Q92854
Application Information	Molecular Weight: Predicted, 96 kDa; observed, 120-140 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB1140 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Semaphorin 4D / CD100
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Semaphorin 4D expression in HeLa cells using anti-Semaphorin 4D antibody (Cat#2500, 1:2,000). Green, isotype control; red, Semaphorin 4D.
Immunocytochemical staining of HeLa cells with anti-Semaphorin 4D antibody (Cat#2500, 1:1,000). Nuclei were stained blue with DAPI; Semaphorin 4D was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Semaphorin 4D antibody (Cat#2500). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Semaphorin 4D antibody (Cat#2500, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

SEMA4D
货号：

2500
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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