Flow cytometric analysis of UCHL1 expression in HAP-1 cells using UCHL1 antibody (Cat#61191, 1:2,000). Green, isotype control; red, UCHL1.
Immunocytochemical staining of HAP-1 cells with UCHL1 antibody (Cat#61191, 1:1,000). Nuclei were stained blue with DAPI; UCHL1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-UCHL1 antibody (Cat#61191). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-UCHL1 antibody (Cat#61191, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). UCHL1, ubiquitin C-terminal hydrolase L1.
Western blotting analysis using anti-UCHL1 antibody (Cat#61191). UCHL1 expression in wild type (WT) and UCHL1 shRNA knockdown (KD) HAP1 cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-UCHL1 antibody (Cat#61191, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of UCHL1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-UCHL1 antibody (Cat#61191, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-UCHL1 antibody (Cat#61191, 1:1,000), Top panel: wild-type (WT); Bottom panal: UCHL1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; UCHL1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
