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Anti-PPP1CA + 1CB Rabbit Monoclonal Antibody #2555

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Flow cytometric analysis of PPP1CA + 1CB expression in HepG2 cells using anti-PPP1CA + 1CB antibody (Cat#2555, 1:2,000). Green, isotype control; red, PPP1CA + 1CB.
Immunocytochemical staining of HepG2 cells with anti-PPP1CA + 1CB antibody (Cat#2555, 1:1,000) . Nuclei were stained blue with DAPI; PPP1CA + 1CB was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-PPP1CA + 1CB antibody (Cat#2555). Total cell lysates (10 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PPP1CA + 1CB antibody (Cat#2555, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    PPP1CA/ PPP1CB
  • 货号:
    2555
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-PPP1CA + 1CB Rabbit Monoclonal Antibody
Aliases PPP1CA; Protein Phosphatase 1 Catalytic Subunit Alpha; PP-1A; PP1alpha; PPP1A; PP1A; Serine/Threonine-Protein Phosphatase PP1-Alpha Catalytic Subunit;
Protein Phosphatase 1, Catalytic Subunit, Alpha Isozyme; EC 3.1.3.16; Serine/Threonine Protein Phosphatase PP1-Alpha 1 Catalytic Subunit; Protein Phosphatase 1, Catalytic Subunit, Alpha Isoform; Testicular Tissue Protein Li 155
Background

Gene Name: PPP1CA/ PPP1CB

NCBI Gene Entry: 5499/5500

UniProt Entry: P62136/P62140

Application Information

Molecular Weight: Predicted, 37 kDa; observed, 35 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB1385

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human PPP1CA + 1CB
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of PPP1CA + 1CB expression in HepG2 cells using anti-PPP1CA + 1CB antibody (Cat#2555, 1:2,000). Green, isotype control; red, PPP1CA + 1CB.
  • Immunocytochemical staining of HepG2 cells with anti-PPP1CA + 1CB antibody (Cat#2555, 1:1,000) . Nuclei were stained blue with DAPI; PPP1CA + 1CB was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-PPP1CA + 1CB antibody (Cat#2555). Total cell lysates (10 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PPP1CA + 1CB antibody (Cat#2555, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of PPP1CA + 1CB expression in HepG2 cells using anti-PPP1CA + 1CB antibody (Cat#2555, 1:2,000). Green, isotype control; red, PPP1CA + 1CB.
Immunocytochemical staining of HepG2 cells with anti-PPP1CA + 1CB antibody (Cat#2555, 1:1,000) . Nuclei were stained blue with DAPI; PPP1CA + 1CB was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-PPP1CA + 1CB antibody (Cat#2555). Total cell lysates (10 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PPP1CA + 1CB antibody (Cat#2555, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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