KD-Validated Anti-ABCB1 Rabbit Monoclonal Antibody - 产品中心

KD-Validated Anti-ABCB1 Rabbit Monoclonal Antibody #61111

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Immunocytochemical staining of HepG2 cells with ABCB1 antibody (Cat#61111, 1:1,000). Nuclei were stained blue with DAPI; ABCB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.

Western blotting analysis using anti-ABCB1 antibody (Cat#61111). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ABCB1 antibody (Cat#61111, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). ABCB1, ATP binding cassette subfamily B member 1.

Western blotting analysis using anti-ABCB1 antibody (Cat#61111). ABCB1 expression in wild type (WT) and ABCB1 shRNA knockdown (KD) Hela cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ABCB1 antibody (Cat#61111, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

ABCB1
货号：

61111
应用：

WB, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information

Product Name	KD-Validated Anti-ABCB1 Rabbit Monoclonal Antibody
Aliases	ABCB1; ATP Binding Cassette Subfamily B Member 1; PGY1; Multidrug Resistance Protein 1; CD243; GP170; ABC20; P-170; MDR1; ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1; ATP-Dependent Translocase ABCB1; Phospholipid Transporter ABCB1; Colchicin Sensitivity; P-Glycoprotein 1; CLCS; P-GP; ATP-Binding Cassette Sub-Family B Member 1; Doxorubicin Resistance; P-Glycoprotein; CD243 Antigen; EC 3.6.3.44; EC 7.6.2.2; EC 7.6.2.1; EC 3.6.3; P-Gp
Background	Gene Name: ABCB1 NCBI Gene Entry: 5243 UniProt Entry: P08183
Application Information	Molecular Weight: Predicted, 141 kDa, observed, 130-180 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB1330 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human P Glycoprotein
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Immunocytochemical staining of HepG2 cells with ABCB1 antibody (Cat#61111, 1:1,000). Nuclei were stained blue with DAPI; ABCB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
Western blotting analysis using anti-ABCB1 antibody (Cat#61111). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ABCB1 antibody (Cat#61111, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). ABCB1, ATP binding cassette subfamily B member 1.
Western blotting analysis using anti-ABCB1 antibody (Cat#61111). ABCB1 expression in wild type (WT) and ABCB1 shRNA knockdown (KD) Hela cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ABCB1 antibody (Cat#61111, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

ABCB1
货号：

61111
应用：

WB, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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