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Anti-SAE1 Rabbit Monoclonal Antibody #2595

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Flow cytometric analysis of SAE1 expression in Hela cells using anti-SAE1 antibody (Cat#2595, 1:2,000). Green, isotype control; red, SAE1.

Immunocytochemical staining of Hela cells with anti-SAE1 antibody (Cat#2595, 1:1,000) . Nuclei were stained blue with DAPI; SAE1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-SAE1 antibody (Cat#2595). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SAE1 antibody (Cat#2595, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

SAE1
货号：

2595
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-SAE1 Rabbit Monoclonal Antibody
Aliases	SUMO1 Activating Enzyme Subunit 1; AOS1; Sua1; Ubiquitin-Like 1-Activating Enzyme E1A; SUMO-Activating Enzyme Subunit 1; FLJ3091; UBLE1A; Ubiquitin-Like Protein SUMO-1 Activating Enzyme; SUMO-1 Activating Enzyme E1 N Subunit; Sentrin/SUMO-Activating Protein AOS1; SUMO-1 Activating Enzyme Subunit 1; Activator Of Sumo 1; Activator Of SUMO1; HSPC140; SUA1
Background	Gene Name: SAE1 NCBI Gene Entry: 10055 UniProt Entry: Q9UBE0
Application Information	Molecular Weight: Predicted, 38 kDa; observed, 36 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB1565 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human SAE1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of SAE1 expression in Hela cells using anti-SAE1 antibody (Cat#2595, 1:2,000). Green, isotype control; red, SAE1.
Immunocytochemical staining of Hela cells with anti-SAE1 antibody (Cat#2595, 1:1,000) . Nuclei were stained blue with DAPI; SAE1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-SAE1 antibody (Cat#2595). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SAE1 antibody (Cat#2595, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

SAE1
货号：

2595
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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