Anti-14-3-3 epsilon Rabbit Monoclonal Antibody - 产品中心

Anti-14-3-3 epsilon Rabbit Monoclonal Antibody #2649

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Flow cytometric analysis of YWHAE expression in HepG2 cells using anti-YWHAE antibody (Cat#2649, 1:2,000). Green, isotype control; red, YWHAE.

Immunocytochemical staining of HepG2 cells with anti-YWHAE antibody (Cat#2649. 1:1,000). Nuclei were stained blue with DAPI; YWHAE was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

Western blotting analysis using anti-YWHAE antibody (Cat#2649). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-YWHAE antibody (Cat#2649, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

YWHAE
货号：

2649
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-14-3-3 epsilon Rabbit Monoclonal Antibody
Aliases	YWHAE; Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein Epsilon; Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein, Epsilon Polypeptide; 14-3-3 Protein Epsilon; 14-3-3 Epsilon; FLJ45465; 14-3-3E; Tyrosine 3/Tryptophan 5 -Monooxygenase Activation Protein, Epsilon Polypeptide; Mitochondrial Import Stimulation Factor L Subunit; Protein Kinase C Inhibitor Protein-1; Epididymis Luminal Protein; KCIP-1; HEL2; MDCR; MDS
Background	Gene Name: YWHAE NCBI Gene Entry: 7531 UniProt Entry: P62258
Application Information	Molecular Weight: Predicted, 29 kDa; observed, 29 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB2120 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human 14-3-3 epsilon
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of YWHAE expression in HepG2 cells using anti-YWHAE antibody (Cat#2649, 1:2,000). Green, isotype control; red, YWHAE.
Immunocytochemical staining of HepG2 cells with anti-YWHAE antibody (Cat#2649. 1:1,000). Nuclei were stained blue with DAPI; YWHAE was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-YWHAE antibody (Cat#2649). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-YWHAE antibody (Cat#2649, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

YWHAE
货号：

2649
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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