Flow cytometric analysis of Gasdermin D expression in HT-1080 cells using anti-Gasdermin D antibody (Cat#4896, 1:2,000). Green, isotype control; red, Gasdermin D.
Immunohistochemistry was performed on paraffin-embedded human endometrial carcinoma using anti-gasdermin D antibody (Cat#4896, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Western blotting analysis using anti-gasdermin D antibody (Cat#4896). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-gasdermin D antibody (Cat#4896, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Applications Tested: Western blotting (WB), flow cytometry (FCM), immunohistochemistry (IHC)
Immunogen
Recombinant protein of human GSDMD
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500 Immunohistochemistry (IHC): 1:50-1:100
Note
This product is for research use only.
Picture
Flow cytometric analysis of Gasdermin D expression in HT-1080 cells using anti-Gasdermin D antibody (Cat#4896, 1:2,000). Green, isotype control; red, Gasdermin D.
Immunohistochemistry was performed on paraffin-embedded human endometrial carcinoma using anti-gasdermin D antibody (Cat#4896, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Western blotting analysis using anti-gasdermin D antibody (Cat#4896). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-gasdermin D antibody (Cat#4896, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Gasdermin D expression in HT-1080 cells using anti-Gasdermin D antibody (Cat#4896, 1:2,000). Green, isotype control; red, Gasdermin D.
Immunohistochemistry was performed on paraffin-embedded human endometrial carcinoma using anti-gasdermin D antibody (Cat#4896, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Western blotting analysis using anti-gasdermin D antibody (Cat#4896). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-gasdermin D antibody (Cat#4896, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
