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Anti-UBE2D1 Rabbit Monoclonal Antibody #2691

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Flow cytometric analysis of UBE2D1 expression in C2C12 cells using anti-UBE2D1 antibody (Cat#2691, 1:2,000). Green, isotype control; red, UBE2D1.

Immunocytochemical staining of C2C12 cells with anti-UBE2D1 antibody (Cat#2691, 1:1,000) . Nuclei were stained blue with DAPI; UBE2D1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-UBE2D1 antibody (Cat#2691). Total cell lysates (5 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-UBE2D1 antibody (Cat#2691, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

UBE2D1
货号：

2691
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

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Information

Product Name	Anti-UBE2D1 Rabbit Monoclonal Antibody
Aliases	UBE2D1; Ubiquitin Conjugating Enzyme E2 D1; UBCH5; Stimulator Of Fe Transport; E2(17)KB1; UbcH5A; UBC4/5; SFT; Ubiquitin-Conjugating Enzyme E2D 1 (UBC4/5 Homolog, Yeast); (E3-Independent) E2 Ubiquitin-Conjugating Enzyme D1; Ubiquitin-Conjugating Enzyme E2-17 KDa 1; Ubiquitin-Conjugating Enzyme E2(17)KB 1; Ubiquitin-Conjugating Enzyme E2 D1; E2 Ubiquitin-Conjugating Enzyme D1; Ubiquitin Carrier Protein D1; Ubiquitin-Protein Ligase D1; UBC4/5 Homolog; Ubiquitin-Conjugating Enzyme E2D 1; Ubiquitin Conjugating Enzyme E2D 1; EC 2.3.2.23; EC 2.3.2.24; EC 6.3.2.19; UBCH5A; UBC5A; UbcH
Background	Gene Name: UBE2D1 NCBI Gene Entry: 7321 UniProt Entry: P51668
Application Information	Molecular Weight: Predicted, 17 kDa; observed, 17 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB1900 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human UBE2D1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of UBE2D1 expression in C2C12 cells using anti-UBE2D1 antibody (Cat#2691, 1:2,000). Green, isotype control; red, UBE2D1.
Immunocytochemical staining of C2C12 cells with anti-UBE2D1 antibody (Cat#2691, 1:1,000) . Nuclei were stained blue with DAPI; UBE2D1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-UBE2D1 antibody (Cat#2691). Total cell lysates (5 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-UBE2D1 antibody (Cat#2691, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

UBE2D1
货号：

2691
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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