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Anti-Desmocollin 3 Rabbit Monoclonal Antibody #2712

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Flow cytometric analysis of Desmocollin 3 expression in HeLa cells using Desmocollin 3 antibody (Cat#2712, 1:2,000). Green, isotype control; red, Desmocollin 3.
Immunocytochemical staining of Hela cells with Desmocollin 3 antibody (Cat#2712, 1:1,000) . Nuclei were stained blue with DAPI; Desmocollin 3 with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Desmocollin 3 antibody (Cat#2712). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Desmocollin 3 antibody (Cat#2712, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    DSC2
  • 货号:
    2712
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-Desmocollin 3 Rabbit Monoclonal Antibody
Aliases DSC3; Desmocollin 3; CDHF3; DSC1; DSC2; DSC4; DSC; Cadherin Family Member 3; Desmocollin-3; Desmocollin-4; HT-CP
Background

Gene Name: DSC2

NCBI Gene Entry: 1824

UniProt Entry: Q02487

Application Information

Molecular Weight: Predicted, 100 kDa; observed, 100 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB1200

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from Desmocollin 3
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Desmocollin 3 expression in HeLa cells using Desmocollin 3 antibody (Cat#2712, 1:2,000). Green, isotype control; red, Desmocollin 3.
  • Immunocytochemical staining of Hela cells with Desmocollin 3 antibody (Cat#2712, 1:1,000) . Nuclei were stained blue with DAPI; Desmocollin 3 with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-Desmocollin 3 antibody (Cat#2712). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Desmocollin 3 antibody (Cat#2712, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of Desmocollin 3 expression in HeLa cells using Desmocollin 3 antibody (Cat#2712, 1:2,000). Green, isotype control; red, Desmocollin 3.
Immunocytochemical staining of Hela cells with Desmocollin 3 antibody (Cat#2712, 1:1,000) . Nuclei were stained blue with DAPI; Desmocollin 3 with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Desmocollin 3 antibody (Cat#2712). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Desmocollin 3 antibody (Cat#2712, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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