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KD-Validated Anti-NDUFB11 Mouse Monoclonal Antibody #64866

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Flow cytometric analysis of NDUFB11 expression in HepG2 cells using anti-NDUFB11 antibody (Cat#64866, 1:2,000). Green, isotype control; red, NDUFB11.
Immunocytochemical staining of HepG2 cells with anti-NDUFB11 associated protein antibody (Cat#64866, 1:1,000) . Nuclei were stained blue with DAPI; NDUFB11 associated protein was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-NDUFB11 antibody (Cat#64866). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NDUFB11 antibody (Cat#64866, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-NDUFB11 antibody (Cat#64866). NDUFB11 expression in wild-type (WT) and NDUFB11 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NDUFB11 antibody (Cat#64866, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with anti-NDUFB11 associated protein antibody (Cat#64866, 1:1,000) . Nuclei were stained blue with DAPI; NDUFB11 associated protein was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • 基因名：
    NDUFB11
  • 货号：
    64866
  • 应用：
    WB, IC, FCM
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-NDUFB11 Mouse Monoclonal Antibody
Aliases NDUFB11; NADH:Ubiquinone Oxidoreductase Subunit B11; NP17.3; Np15; NADH Dehydrogenase [Ubiquinone] 1 Beta Subcomplex Subunit 11, Mitochondrial; NADH Dehydrogenase (Ubiquinone) 1 Beta Subcomplex, 11, 17.3kDa; NADH-Ubiquinone Oxidoreductase ESSS Subunit; Complex I NP17.3 Subunit; Neuronal Protein 17.3; Complex I-ESSS; CI-ESSS; P17.3; MC1DN30; Np17.3
Background

Gene Name: NDUFB11

NCBI Gene Entry: 54539

UniProt Entry: Q9NX14
Application Information

Molecular Weight: Predicted, 17 kDa; observed, 18 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB13335

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen Recombinant protein of human NDUFB11
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of NDUFB11 expression in HepG2 cells using anti-NDUFB11 antibody (Cat#64866, 1:2,000). Green, isotype control; red, NDUFB11.
  • Immunocytochemical staining of HepG2 cells with anti-NDUFB11 associated protein antibody (Cat#64866, 1:1,000) . Nuclei were stained blue with DAPI; NDUFB11 associated protein was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-NDUFB11 antibody (Cat#64866). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NDUFB11 antibody (Cat#64866, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-NDUFB11 antibody (Cat#64866). NDUFB11 expression in wild-type (WT) and NDUFB11 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NDUFB11 antibody (Cat#64866, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with anti-NDUFB11 associated protein antibody (Cat#64866, 1:1,000) . Nuclei were stained blue with DAPI; NDUFB11 associated protein was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Citation(0)
  • 基因名：
    NDUFB11
  • 货号：
    64866
  • 应用：
    WB, IC, FCM
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of NDUFB11 expression in HepG2 cells using anti-NDUFB11 antibody (Cat#64866, 1:2,000). Green, isotype control; red, NDUFB11.
Immunocytochemical staining of HepG2 cells with anti-NDUFB11 associated protein antibody (Cat#64866, 1:1,000) . Nuclei were stained blue with DAPI; NDUFB11 associated protein was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-NDUFB11 antibody (Cat#64866). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NDUFB11 antibody (Cat#64866, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-NDUFB11 antibody (Cat#64866). NDUFB11 expression in wild-type (WT) and NDUFB11 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NDUFB11 antibody (Cat#64866, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with anti-NDUFB11 associated protein antibody (Cat#64866, 1:1,000) . Nuclei were stained blue with DAPI; NDUFB11 associated protein was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
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