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Anti-Ubiquitin Like Modifier Activating Enzyme 1 Rabbit Monoclonal Antibody #2729

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Flow cytometric analysis of Ubiquitin like modifier activating enzyme 1 expression in HepG2 cells using anti-Ubiquitin like modifier activating enzyme 1 antibody (Cat#2729, 1:2,000). Green, isotype control; red, Ubiquitin like modifier activating enzyme 1.
Immunocytochemical staining of HepG2 cells with anti-Ubiquitin like modifier activating enzyme 1 antibody (Cat#2729, 1:1.000). Nuclei were stained blue with DAPI; Ubiquitin like modifier activating enzyme 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Ubiquitin like modifier activating enzyme 1 antibody (Cat#2729). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Ubiquitin like modifier activating enzyme 1 antibody (Cat#2729, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    UBA1
  • 货号：
    2729
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-Ubiquitin Like Modifier Activating Enzyme 1 Rabbit Monoclonal Antibody
Aliases UBA1; Ubiquitin Like Modifier Activating Enzyme 1; CFAP124; UBE1X; POC20; A1S9T; UBE1; Ubiquitin-Like Modifier-Activating Enzyme 1; Ubiquitin-Activating Enzyme E1; GXP1; Ubiquitin-Activating Enzyme E1 (A1S9T And BN75 Temperature Sensitivity Complementing); UBA1, Ubiquitin-Activating Enzyme E1 Homolog (Yeast); A1S9T And BN75 Temperature Sensitivity Complementing; POC20 Centriolar Protein Homolog (Chlamydomonas); UBA1, Ubiquitin-Activating Enzyme E1 Homolog A; Ubiquitin-Like Modifier Activating Enzyme 1; Testicular Secretory Protein Li 63; POC20 Centriolar Protein Homolog; Protein A1S9; EC 6.2.1.45; AMCX1; SMAX2; UBA1A; VEXAS; A1S9; A1ST
Background

Gene Name: UBA1

NCBI Gene Entry: 7317

UniProt Entry: P22314
Application Information

Molecular Weight: Predicted, 118 kDa; observed, 118 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB2435

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human E1 Ubiquitin Activating Enzyme
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Ubiquitin like modifier activating enzyme 1 expression in HepG2 cells using anti-Ubiquitin like modifier activating enzyme 1 antibody (Cat#2729, 1:2,000). Green, isotype control; red, Ubiquitin like modifier activating enzyme 1.
  • Immunocytochemical staining of HepG2 cells with anti-Ubiquitin like modifier activating enzyme 1 antibody (Cat#2729, 1:1.000). Nuclei were stained blue with DAPI; Ubiquitin like modifier activating enzyme 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-Ubiquitin like modifier activating enzyme 1 antibody (Cat#2729). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Ubiquitin like modifier activating enzyme 1 antibody (Cat#2729, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    UBA1
  • 货号：
    2729
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Flow cytometric analysis of Ubiquitin like modifier activating enzyme 1 expression in HepG2 cells using anti-Ubiquitin like modifier activating enzyme 1 antibody (Cat#2729, 1:2,000). Green, isotype control; red, Ubiquitin like modifier activating enzyme 1.
Immunocytochemical staining of HepG2 cells with anti-Ubiquitin like modifier activating enzyme 1 antibody (Cat#2729, 1:1.000). Nuclei were stained blue with DAPI; Ubiquitin like modifier activating enzyme 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Ubiquitin like modifier activating enzyme 1 antibody (Cat#2729). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Ubiquitin like modifier activating enzyme 1 antibody (Cat#2729, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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