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Anti-BRCC3 Rabbit Monoclonal Antibody #5443

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Flow cytometric analysis of BRCC3 expression in HepG2 cells using anti-BRCC3 antibody (Cat#5443, 1:2,000). Green, isotype control; red, BRCC3.

Immunocytochemical staining of HepG2 cells with anti-BRCC3 antibody (Cat#5443, 1:1,000) . Nuclei were stained blue with DAPI; BRCC3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-BRCC3 antibody (Cat#5443). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-BRCC3 antibody (Cat#5443, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

BRCC3
货号：

5443
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-BRCC3 Rabbit Monoclonal Antibody
Aliases	BRCC3; BRCA1/BRCA2-Containing Complex Subunit 3; BRCC36; C6.1A; CXorf53; BRCA1/BRCA2-Containing Complex Subunit 36; Lys-63-Specific Deubiquitinase BRCC36; BRCA1-A Complex Subunit BRCC36; BRISC Complex Subunit BRCC36; BRCA1/BRCA2-Containing Complex, Subunit 3; Chromosome X Open Reading Frame 53; Lys-63-Specific Deubiquitinase; EC 3.4.19.-; EC 3.1.2.15
Background	Gene Name: BRCC3 NCBI Gene Entry: 79184 UniProt Entry: P46736
Application Information	Molecular Weight: Predicted, 36 kDa; observed, 32 kDa Clonality: Rabbit monoclonal antibody Clone ID: 25GB1580 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human BRCC3
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of BRCC3 expression in HepG2 cells using anti-BRCC3 antibody (Cat#5443, 1:2,000). Green, isotype control; red, BRCC3.
Immunocytochemical staining of HepG2 cells with anti-BRCC3 antibody (Cat#5443, 1:1,000) . Nuclei were stained blue with DAPI; BRCC3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-BRCC3 antibody (Cat#5443). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-BRCC3 antibody (Cat#5443, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

BRCC3
货号：

5443
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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