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Anti-ALPP Mouse Monoclonal Antibody #2836

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Immunocytochemical staining of HepG2 cells with anti-ALPP antibody (Cat#2836, 1:1,000). Nuclei were stained blue with DAPI; ALPP was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
Western blotting analysis using anti-ALPP antibody (Cat#2836). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ALPP antibody (Cat#2836, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    ALPP
  • 货号：
    2836
  • 应用：
    WB, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-ALPP Mouse Monoclonal Antibody
Aliases ALPP; Alkaline Phosphatase, Placental; PLAP; PALP; Alkaline Phosphatase, Placental Type; Alkaline Phosphatase Regan Isozyme; Placental Alkaline Phosphatase 1; EC 3.1.3.1; PLAP-1; Alkaline Phosphatase, Placental (Regan Isozyme); Intestinal-Type Alkaline Phosphatase; Intestinal Alkaline Phosphatase; Placental Alkaline Phosphatase; Alkaline Phosphomonoesterase; Glycerophosphatase; Regan Isozyme; ALPI; ALP; IAP
Background

Gene Name: ALPP

NCBI Gene Entry: 250

UniProt Entry: P05187
Application Information

Molecular Weight: Predicted, 58 kDa; observed, 70 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB2770

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human ALPP
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,500-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Immunocytochemical staining of HepG2 cells with anti-ALPP antibody (Cat#2836, 1:1,000). Nuclei were stained blue with DAPI; ALPP was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
  • Western blotting analysis using anti-ALPP antibody (Cat#2836). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ALPP antibody (Cat#2836, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    ALPP
  • 货号：
    2836
  • 应用：
    WB, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Immunocytochemical staining of HepG2 cells with anti-ALPP antibody (Cat#2836, 1:1,000). Nuclei were stained blue with DAPI; ALPP was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
Western blotting analysis using anti-ALPP antibody (Cat#2836). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ALPP antibody (Cat#2836, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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