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Anti-Vimentin Mouse Monoclonal Antibody #2839

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Flow cytometric analysis of vimentin expression in C2C12 cells using anti-vimentin antibody (Cat#2839, 1:2,000). Green, isotype control; red, vimentin.

Immunocytochemical staining of Hela cells with vimentin antibody (Cat#2839, 1:1,000). Nuclei were stained blue with DAPI; Vimentin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

Immunohistochemistry was performed on paraffin-embedded human endometrial carcinoma using anti-vimentin antibody (Cat#2839, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.

Western blotting analysis using anti-Vimentin antibody (Cat#2839). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Vimentin antibody (Cat#2839, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

VIM
货号：

2839
应用：

WB, FCM, IC, IHC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-Vimentin Mouse Monoclonal Antibody
Aliases	VIM; Vimentin; Epididymis Secretory Sperm Binding Protein
Background	Gene Name: VIM NCBI Gene Entry: 7431 UniProt Entry: P08670
Application Information	Molecular Weight: Predicted, 54 kDa; observed, 56 kDa Clonality: Mouse monoclonal antibody Clone ID: 24GB2785 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC), immunohistochemistry (IHC)
Immunogen	Recombinant protein of human Vimentin
Isotype	Mouse IgM kappa
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:2,500-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000 Flow Cytometry (FCM) : 1:2,000 Immunohistochemistry (IHC): 1:50-1:100
Note	This product is for research use only.

Picture

Flow cytometric analysis of vimentin expression in C2C12 cells using anti-vimentin antibody (Cat#2839, 1:2,000). Green, isotype control; red, vimentin.
Immunocytochemical staining of Hela cells with vimentin antibody (Cat#2839, 1:1,000). Nuclei were stained blue with DAPI; Vimentin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Immunohistochemistry was performed on paraffin-embedded human endometrial carcinoma using anti-vimentin antibody (Cat#2839, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Western blotting analysis using anti-Vimentin antibody (Cat#2839). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Vimentin antibody (Cat#2839, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

VIM
货号：

2839
应用：

WB, FCM, IC, IHC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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