KD-Validated Anti-Grp75 Rabbit Monoclonal Antibody - 产品中心

KD-Validated Anti-Grp75 Rabbit Monoclonal Antibody #61525

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Immunocytochemical staining of HepG2 cells with Grp75 antibody (Cat#61525, 1:1,000). Nuclei were stained blue with DAPI; Grp75 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-Grp75 antibody (Cat#61525). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Grp75 antibody (Cat#61525, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis using anti-Grp75 antibody (Cat#61525). Grp75 expression in wild type (WT) and Grp75 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Grp75 antibody (Cat#61525, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

HSPA9
货号：

61525
应用：

WB, IC
物种反应性：

H, M

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information

Product Name	KD-Validated Anti-Grp75 Rabbit Monoclonal Antibody
Aliases	HSPA9; Heat Shock Protein Family A (Hsp70) Member 9; GRP75; PBP74; 75 KDa Glucose-Regulated Protein; Stress-70 Protein, Mitochondrial; HSPA9B; Heat Shock 70kDa Protein 9 (Mortalin); Peptide-Binding Protein 74; Mortalin2; Mortalin; MTHSP75; GRP-75; MOT; Epididymis Secretory Sperm Binding Protein Li 124m; Heat Shock 70kDa Protein 9B (Mortalin-2); Catecholamine-Regulated Protein 40; Heat Shock 70 KDa Protein 9; Heat Shock 70kD Protein 9B; Mortalin, Perinuclear; P66-Mortalin; HEL-S-124m; Mortalin-2; Mt-HSP70; Mthsp75; SIDBA4; Mot-2; CRP40; EVPLS; MOT-2; MOT2; SAAN; CSA
Background	Gene Name: HSPA9 NCBI Gene Entry: 3313 UniProt Entry: P38646
Application Information	Molecular Weight: Predicted, 74 kDa, observed, 74 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB2615 Species Reactivity: Human, mouse Applications Tested: Western blotting (WB), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Grp75
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Immunocytochemical staining of HepG2 cells with Grp75 antibody (Cat#61525, 1:1,000). Nuclei were stained blue with DAPI; Grp75 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Grp75 antibody (Cat#61525). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Grp75 antibody (Cat#61525, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Grp75 antibody (Cat#61525). Grp75 expression in wild type (WT) and Grp75 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Grp75 antibody (Cat#61525, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

HSPA9
货号：

61525
应用：

WB, IC
物种反应性：

H, M

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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