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Anti-Keratin 8 Rabbit Monoclonal Antibody #2857

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Flow cytometric analysis of Keratin 8 expression in HepG2 cells using anti-Keratin 8 antibody (Cat#2857, 1:2,000). Green, isotype control; red, Keratin 8.

Immunocytochemical staining of HepG2 cells with anti-Keratin 8 antibody(Cat#2857, 1:1,000) . Nuclei were stained blue with DAPI; Keratin 8 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using anti-Keratin 8 antibody (Cat#2857). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Keratin 8 antibody (Cat#2857, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

KRT8
货号：

2857
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

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Citation(0)

Information

Product Name	Anti-Keratin 8 Rabbit Monoclonal Antibody
Aliases	KRT8; Keratin 8; CK-8; CYK8; K8; CARD2; K2C8; CK8; KO; Keratin, Type II Cytoskeletal 8; Type-II Keratin Kb8; Keratin 8, Type II; Cytokeratin-8; Keratin-8
Background	Gene Name: KRT8 NCBI Gene Entry: 3856 UniProt Entry: P05787
Application Information	Molecular Weight: Predicted, 54 kDa; observed, 48 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB2865 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	Recombinant protein of human Cytokeratin 8
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Keratin 8 expression in HepG2 cells using anti-Keratin 8 antibody (Cat#2857, 1:2,000). Green, isotype control; red, Keratin 8.
Immunocytochemical staining of HepG2 cells with anti-Keratin 8 antibody(Cat#2857, 1:1,000) . Nuclei were stained blue with DAPI; Keratin 8 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-Keratin 8 antibody (Cat#2857). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Keratin 8 antibody (Cat#2857, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

KRT8
货号：

2857
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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