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Anti-HLA-B Mouse Monoclonal Antibody #2881

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Immunocytochemical staining of HAP-1 cells with anti-HLA-B antibody (Cat#2881, 1:1,000) . Nuclei were stained blue with DAPI; HLA-B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：Medium. Scale bar, 20 μm.
Western blotting analysis using anti-HLA-B antibody (Cat#2881). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HLA-B antibody (Cat#2881, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    HLA-B
  • 货号：
    2881
  • 应用：
    WB, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-HLA-B Mouse Monoclonal Antibody
Aliases HLA-B; Major Histocompatibility Complex, Class I, B; HLA Class I Histocompatibility Antigen, B Alpha Chain; HLAB; AS; MHC Class I Antigen HLA-B Alpha Chain; MHC Class I Antigen HLA-B Heavy Chain; MHC HLA-B Transmembrane Glycoprotein; MHC HLA-B Cell Surface Glycoprotein; Leukocyte Antigen Class I-B; HLA Class I Antigen HLA-B; MHC Class I Antigen SHCHA; Human Leukocyte Antigen B; Ankylosing Spondylitis; MHC Class I Molecule; MHC Class 1 Antigen; B-4901
Background

Gene Name: HLA-B

NCBI Gene Entry: 3106

UniProt Entry: P01889
Application Information

Molecular Weight: Predicted, 40 kDa; observed, 38 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB2980

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human HLA-B
Isotype Mouse IgM
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,500-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Immunocytochemical staining of HAP-1 cells with anti-HLA-B antibody (Cat#2881, 1:1,000) . Nuclei were stained blue with DAPI; HLA-B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-HLA-B antibody (Cat#2881). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HLA-B antibody (Cat#2881, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    HLA-B
  • 货号：
    2881
  • 应用：
    WB, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Immunocytochemical staining of HAP-1 cells with anti-HLA-B antibody (Cat#2881, 1:1,000) . Nuclei were stained blue with DAPI; HLA-B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：Medium. Scale bar, 20 μm.
Western blotting analysis using anti-HLA-B antibody (Cat#2881). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HLA-B antibody (Cat#2881, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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