Anti-Hepatocyte Nuclear Factor 4 Alpha Rabbit Monoclonal Antibody - 产品中心

Anti-Hepatocyte Nuclear Factor 4 Alpha Rabbit Monoclonal Antibody #2894

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Flow cytometric analysis of Hepatocyte nuclear factor 4 alpha expression in HepG2 cells using anti-Hepatocyte nuclear factor 4 alpha antibody (Cat#2894, 1:2,000). Green, isotype control; red, Hepatocyte nuclear factor 4 alpha.

Immunocytochemical staining of HepG2 cells with anti-Hepatocyte nuclear factor 4 alpha antibody (Cat#2894, 1:1000) . Nuclei were stained blue with DAPI;Hepatocyte nuclear factor 4 alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Meidum . Scale bar, 20 μm.

Western blotting analysis using anti-Hepatocyte nuclear factor 4 alpha antibody (Cat#2894). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Hepatocyte nuclear factor 4 alpha antibody (Cat#2894, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

HNF4A
货号：

2894
应用：

WB, FCM, IC
物种反应性：

H, M

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-Hepatocyte Nuclear Factor 4 Alpha Rabbit Monoclonal Antibody
Aliases	HNF4A; Hepatocyte Nuclear Factor 4 Alph; NR2A1; HNF4; TCF14; Nuclear Receptor Subfamily 2 Group A Member 1; Hepatocyte Nuclear Factor 4-Alpha; Transcription Factor HNF-; Transcription Factor 14; TCF-14; MODY1; MODY; Hepatic Nuclear Factor 4 Alpha; HNF4alpha10/11/12; HNF4A Wild Type; HNF-4-Alpha; HNF4alph; HNF4a7; HNF4a8; HNF4a9; NR2A21; FRTS4; TCF
Background	Gene Name: HNF4A NCBI Gene Entry: 3172 UniProt Entry: P41235
Application Information	Molecular Weight: Predicted, 53 kDa; observed, 50 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB3045 Species Reactivity: Human, mouse Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human HNF-4-alpha
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Hepatocyte nuclear factor 4 alpha expression in HepG2 cells using anti-Hepatocyte nuclear factor 4 alpha antibody (Cat#2894, 1:2,000). Green, isotype control; red, Hepatocyte nuclear factor 4 alpha.
Immunocytochemical staining of HepG2 cells with anti-Hepatocyte nuclear factor 4 alpha antibody (Cat#2894, 1:1000) . Nuclei were stained blue with DAPI;Hepatocyte nuclear factor 4 alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Meidum . Scale bar, 20 μm.
Western blotting analysis using anti-Hepatocyte nuclear factor 4 alpha antibody (Cat#2894). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Hepatocyte nuclear factor 4 alpha antibody (Cat#2894, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

HNF4A
货号：

2894
应用：

WB, FCM, IC
物种反应性：

H, M

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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