Flow cytometric analysis of SRSF9 expression in HeLa cells using anti-SRSF9 antibody (Cat#63578, 1:2,000). Green, isotype control; red, SRSF9.
Immunocytochemical staining of HeLa cells with anti-SRSF9 antibody (Cat#63578, 1:1,000) . Nuclei were stained blue with DAPI; SRSF9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain:Low. Scale bar, 20 μm.
Western blotting analysis using anti-SRSF9 antibody (Cat#63578). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SRSF9 antibody (Cat#63578, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-SRSF9 antibody (Cat#63578). SRSF9 expression in wild type (WT) and SRSF9 knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-SRSF9 antibody (Cat#63578, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).