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Anti-Enolase 1 Rabbit Monoclonal Antibody #2911

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Flow cytometric analysis of Enolase 1 expression in HepG2 cells using anti-Enolase 1 antibody (Cat#2911, 1:2,000). Green, isotype control; red, Enolase 1.
Immunocytochemical staining of HepG2 cells with anti-Enolase 1 antibody (Cat#2911, 1:1,000). Nuclei were stained blue with DAPI; Enolase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Enolase 1 antibody (Cat#2911). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Enolase 1 antibody (Cat#2911, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    ENO1
  • 货号：
    2911
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-Enolase 1 Rabbit Monoclonal Antibody
Aliases ENO1; Enolase 1; Alpha-Enolase; ENO1L1; MBP-1; MPB1; PPH; ENO1 Intronic Transcript 1 (Non-Protein Coding); 2-Phospho-D-Glycerate Hydro-Lyase; Plasminogen-Binding Protein; ENO1 Intronic Transcript 1; Phosphopyruvate Hydratase; Enolase 1, (Alpha); Non-Neural Enolase; EC 4.2.1.11; ENO1-IT1; NNE; Epididymis Secretory Protein Li 17; C-Myc Promoter-Binding Protein-1; MYC Promoter-Binding Protein 1; C-Myc Promoter-Binding Protein; Alpha Enolase Like 1; Tau-Crystallin; Enolase-Alpha; HEL-S-17; EC 4.2.1; MBPB1; MPB-1
Background

Gene Name: ENO1

NCBI Gene Entry: 2023

UniProt Entry: P06733
Application Information

Molecular Weight: Predicted, 47 kDa; observed, 47 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB3130

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human ENO1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Enolase 1 expression in HepG2 cells using anti-Enolase 1 antibody (Cat#2911, 1:2,000). Green, isotype control; red, Enolase 1.
  • Immunocytochemical staining of HepG2 cells with anti-Enolase 1 antibody (Cat#2911, 1:1,000). Nuclei were stained blue with DAPI; Enolase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-Enolase 1 antibody (Cat#2911). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Enolase 1 antibody (Cat#2911, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    ENO1
  • 货号：
    2911
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Flow cytometric analysis of Enolase 1 expression in HepG2 cells using anti-Enolase 1 antibody (Cat#2911, 1:2,000). Green, isotype control; red, Enolase 1.
Immunocytochemical staining of HepG2 cells with anti-Enolase 1 antibody (Cat#2911, 1:1,000). Nuclei were stained blue with DAPI; Enolase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Enolase 1 antibody (Cat#2911). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Enolase 1 antibody (Cat#2911, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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