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Anti-Fibroblast Growth Factor Receptor 1 Rabbit Monoclonal Antibody #2934

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Flow cytometric analysis of Fibroblast growth factor receptor 1 expression in HAP-1 cells using anti-Fibroblast growth factor receptor 1 antibody (Cat#2934, 1:2,000). Green, isotype control; red, Fibroblast growth factor receptor 1.
Immunocytochemical staining of HAP-1 with anti-Fibroblast growth factor receptor 1 antibody (Cat#2934, 1:1,000) . Nuclei were stained blue with DAPI; Fibroblast growth factor receptor 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Fibroblast growth factor receptor 1 antibody (Cat#2934). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Fibroblast growth factor receptor 1 antibody (Cat#2934, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    FGFR1
  • 货号:
    2934
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-Fibroblast Growth Factor Receptor 1 Rabbit Monoclonal Antibody
Aliases FGFR1; Fibroblast Growth Factor Receptor 1; BFGFR; CEK; FLG; N-SAM; CD331; FLT2; Basic Fibroblast Growth Factor Receptor 1; Fms-Related Tyrosine Kinase 2; Proto-Oncogene C-Fgr; EC 2.7.10.1; BFGF-R-1; FGFR-1; FGFBR; FLT-2; HBGFR; KAL2; H2; H3; H4; H5; Heparin-Binding Growth Factor Receptor; FMS-Like Tyrosine Kinase 2; Hydroxyaryl-Protein Kinase; Fms-Like Tyrosine Kinase 2; FGFR1/PLAG1 Fusion; Pfeiffer Syndrome; CD331 Antigen; EC 2.7.10; HRTFDS; N-Sam; ECCL; HH2; OGD
Background

Gene Name: FGFR1

NCBI Gene Entry: 2260

UniProt Entry: P11362

Application Information

Molecular Weight: Predicted, 92 kDa; observed, 150 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB3240

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human FGFR1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Fibroblast growth factor receptor 1 expression in HAP-1 cells using anti-Fibroblast growth factor receptor 1 antibody (Cat#2934, 1:2,000). Green, isotype control; red, Fibroblast growth factor receptor 1.
  • Immunocytochemical staining of HAP-1 with anti-Fibroblast growth factor receptor 1 antibody (Cat#2934, 1:1,000) . Nuclei were stained blue with DAPI; Fibroblast growth factor receptor 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-Fibroblast growth factor receptor 1 antibody (Cat#2934). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Fibroblast growth factor receptor 1 antibody (Cat#2934, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of Fibroblast growth factor receptor 1 expression in HAP-1 cells using anti-Fibroblast growth factor receptor 1 antibody (Cat#2934, 1:2,000). Green, isotype control; red, Fibroblast growth factor receptor 1.
Immunocytochemical staining of HAP-1 with anti-Fibroblast growth factor receptor 1 antibody (Cat#2934, 1:1,000) . Nuclei were stained blue with DAPI; Fibroblast growth factor receptor 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Fibroblast growth factor receptor 1 antibody (Cat#2934). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Fibroblast growth factor receptor 1 antibody (Cat#2934, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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