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Anti-Integrin alpha 5 Rabbit Monoclonal Antibody #4722

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Immunocytochemical staining of C2C12 cells with anti-ITGA5 antibody(Cat#4722, 1:1,000) . Nuclei were stained blue with DAPI; ITGA5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Flow cytometric analysis of integrin alpha 5 expression in C2C12 cells using anti-integrin alpha 5 antibody (Cat#4722, 1:2,000). Green, isotype control; red, integrin alpha 5.

Western blotting analysis using anti-integrin alpha 5 antibody (Cat#4722). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-integrin alpha 5 antibody (Cat#4722, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

ITGA5
货号：

4722
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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Picture

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Information

Product Name	Anti-Integrin alpha 5 Rabbit Monoclonal Antibody
Aliases	Integrin; Alpha 5 (Fibronectin Receptor,Alpha Polypeptide); VLA5A FNRA; CD49e; Fibronectin Receptor Subunit Alpha; Fibronectin Receptor Alpha Subunit; Integrin Alpha-F; Integrin Alpha-5; VLA-5; Very Late Activation Protein 5; Alpha Subunit; CD49 Antigen-Like Family Member E; CD49e Antigen
Background	Gene Name: ITGA5 NCBI Gene Entry: 3678 UniProt Entry: P08648
Application Information	Molecular Weight: Predicted, 115 kDa; observed, 150 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB12620 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Integrin alpha 5
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Immunocytochemical staining of C2C12 cells with anti-ITGA5 antibody(Cat#4722, 1:1,000) . Nuclei were stained blue with DAPI; ITGA5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of integrin alpha 5 expression in C2C12 cells using anti-integrin alpha 5 antibody (Cat#4722, 1:2,000). Green, isotype control; red, integrin alpha 5.
Western blotting analysis using anti-integrin alpha 5 antibody (Cat#4722). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-integrin alpha 5 antibody (Cat#4722, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

ITGA5
货号：

4722
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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