Anti-NEDD4L Rabbit Monoclonal Antibody - 产品中心 - 合肥善本生物科技有限公司

Anti-NEDD4L Rabbit Monoclonal Antibody #2942

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Flow cytometric analysis of NEDD4L expression in HepG2 cells using anti-NEDD4L antibody (Cat#2942, 1:2,000). Green, isotype control; red, NEDD4L.

Immunocytochemical staining of HepG2 cells with anti-NEDD4L antibody (Cat#2942, 1:1,000). Nuclei were stained blue with DAPI; NEDD4L was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-NEDD4L antibody (Cat#2942). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NEDD4L antibody (Cat#2942, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

NEDD4L
货号：

2942
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-NEDD4L Rabbit Monoclonal Antibody
Aliases	NEDD4L; NEDD4 Like E3 Ubiquitin Protein Ligase; KIAA0439; NEDD4-2; RSP5; Neural Precursor Cell Expressed, Developmentally Down-Regulated 4-Like, E3 Ubiquitin Protein Ligase; HECT-Type E3 Ubiquitin Transferase NED4L; E3 Ubiquitin-Protein Ligase NEDD4-Like; NEDD4.2; Neural Precursor Cell Expressed, Developmentally Down-Regulated 4-Like; Ubiquitin-Protein Ligase Rsp5; EC 2.3.2.26; EC 2.3.2.36; HNEDD4-2; EC 6.3.2; Nedd4-2; PVNH7; NEDL3
Background	Gene Name: NEDD4L NCBI Gene Entry: 23327 UniProt Entry: Q96PU5
Application Information	Molecular Weight: Predicted, 112 kDa; observed, 130 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB3280 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human NEDD4-2
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:4,000-1:20,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of NEDD4L expression in HepG2 cells using anti-NEDD4L antibody (Cat#2942, 1:2,000). Green, isotype control; red, NEDD4L.
Immunocytochemical staining of HepG2 cells with anti-NEDD4L antibody (Cat#2942, 1:1,000). Nuclei were stained blue with DAPI; NEDD4L was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-NEDD4L antibody (Cat#2942). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NEDD4L antibody (Cat#2942, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

NEDD4L
货号：

2942
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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