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Anti-HTR Rabbit Monoclonal Antibody #2979

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Flow cytometric analysis of 5-hydroxytryptamine receptor 2C expression in HepG2 cells using anti-5-hydroxytryptamine receptor 2C antibody (Cat#2979, 1:2,000). Green, isotype control; red, 5-hydroxytryptamine receptor 2C.
Immunocytochemical staining of HepG2 cells with anti-5-hydroxytryptamine receptor 2C antibody (Cat#2979, 1:1,000) . Nuclei were stained blue with DAPI; 5-hydroxytryptamine receptor 2C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-5-hydroxytryptamine receptor 2C antibody (Cat#2979). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-5-hydroxytryptamine receptor 2C antibody (Cat#2979, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    HTR2C
  • 货号:
    2979
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-HTR Rabbit Monoclonal Antibody
Aliases HTR; 5-Hydroxytryptamine Receptor 2C; 5-HT2C; 5HTR2C; HTR1C; 5-Hydroxytryptamine (Serotonin) Receptor 2C, G Protein-Coupled; 5-Hydroxytryptamine Receptor 1C; 5-HTR2C; 5-HT1C; 5-Hydroxytryptamine (Serotonin) Receptor 2C; Serotonin 5-HT-1C Receptor; Serotonin 5-HT-2C Receptor; Serotonin Receptor 2C; 5-HT-2C; 5-HT-1C
Background

Gene Name: HTR2C

NCBI Gene Entry: 3358

UniProt Entry: P28335

Application Information

Molecular Weight: Predicted, 52 kDa; observed, 73 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB3460

Species Reactivity: Human, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human 5HT2C Receptor
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of 5-hydroxytryptamine receptor 2C expression in HepG2 cells using anti-5-hydroxytryptamine receptor 2C antibody (Cat#2979, 1:2,000). Green, isotype control; red, 5-hydroxytryptamine receptor 2C.
  • Immunocytochemical staining of HepG2 cells with anti-5-hydroxytryptamine receptor 2C antibody (Cat#2979, 1:1,000) . Nuclei were stained blue with DAPI; 5-hydroxytryptamine receptor 2C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
  • Western blotting analysis using anti-5-hydroxytryptamine receptor 2C antibody (Cat#2979). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-5-hydroxytryptamine receptor 2C antibody (Cat#2979, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of 5-hydroxytryptamine receptor 2C expression in HepG2 cells using anti-5-hydroxytryptamine receptor 2C antibody (Cat#2979, 1:2,000). Green, isotype control; red, 5-hydroxytryptamine receptor 2C.
Immunocytochemical staining of HepG2 cells with anti-5-hydroxytryptamine receptor 2C antibody (Cat#2979, 1:1,000) . Nuclei were stained blue with DAPI; 5-hydroxytryptamine receptor 2C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-5-hydroxytryptamine receptor 2C antibody (Cat#2979). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-5-hydroxytryptamine receptor 2C antibody (Cat#2979, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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