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KD-Validated Anti-Phospho-BCAR1 (Y410) Rabbit Monoclonal Antibody #61636

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Flow cytometric analysis of Phospho-BCAR1 (Y410) expression in C2C12 cells using Phospho-BCAR1 (Y410) antibody (Cat#61636, 1:2,000). Green, isotype control; red, Phospho-BCAR1 (Y410).
Immunocytochemical staining of HepG2 cells with anti-Phospho-BCAR1 (Y410) antibody (Cat#61636, 1:1,000) . Nuclei were stained blue with anti-DAPI; Phospho-BCAR1 (Y410) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-Phospho-BCAR1 (Y410) antibody (Cat#61636). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-BCAR1 (Y410) antibody (Cat#61636, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Phospho-BCAR1 (Y410) antibody (Cat#61636). Phospho-BCAR1 (Y410) expression in wild type (WT) and BCAR1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Phospho-BCAR1 (Y410) antibody (Cat#61636, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    BCAR1
  • 货号：
    61636
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-Phospho-BCAR1 (Y410) Rabbit Monoclonal Antibody
Aliases BCAR1; BCAR1 Scaffold Protein, Cas Family Member; CASS1; CAS; Cas Scaffolding Protein Family Member 1; CRKAS; Breast Cancer Anti-Estrogen Resistance Protein 1; BCAR1, Cas Family Scaffolding Protein; BCAR1, Cas Family Scaffold Protein; P130Cas; Breast Cancer Anti-Estrogen Resistance 1; Crk-Associated Substrate P130Cas; Crk-Associated Substrate; CRK-Associated Substrate; P130CAS; P130cas; Crkas; CAS1
Background

Gene Name: BCAR1

NCBI Gene Entry: 9564

UniProt Entry: P56945
Application Information

Molecular Weight: Predicted, 93 kDa, observed, 130 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB3870

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human Phospho-BCAR1 (Y410)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Phospho-BCAR1 (Y410) expression in C2C12 cells using Phospho-BCAR1 (Y410) antibody (Cat#61636, 1:2,000). Green, isotype control; red, Phospho-BCAR1 (Y410).
  • Immunocytochemical staining of HepG2 cells with anti-Phospho-BCAR1 (Y410) antibody (Cat#61636, 1:1,000) . Nuclei were stained blue with anti-DAPI; Phospho-BCAR1 (Y410) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
  • Western blotting analysis using anti-Phospho-BCAR1 (Y410) antibody (Cat#61636). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-BCAR1 (Y410) antibody (Cat#61636, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-Phospho-BCAR1 (Y410) antibody (Cat#61636). Phospho-BCAR1 (Y410) expression in wild type (WT) and BCAR1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Phospho-BCAR1 (Y410) antibody (Cat#61636, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    BCAR1
  • 货号：
    61636
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of Phospho-BCAR1 (Y410) expression in C2C12 cells using Phospho-BCAR1 (Y410) antibody (Cat#61636, 1:2,000). Green, isotype control; red, Phospho-BCAR1 (Y410).
Immunocytochemical staining of HepG2 cells with anti-Phospho-BCAR1 (Y410) antibody (Cat#61636, 1:1,000) . Nuclei were stained blue with anti-DAPI; Phospho-BCAR1 (Y410) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-Phospho-BCAR1 (Y410) antibody (Cat#61636). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-BCAR1 (Y410) antibody (Cat#61636, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Phospho-BCAR1 (Y410) antibody (Cat#61636). Phospho-BCAR1 (Y410) expression in wild type (WT) and BCAR1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Phospho-BCAR1 (Y410) antibody (Cat#61636, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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