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Anti-ATM Rabbit Monoclonal Antibody #2994

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Flow cytometric analysis of ATM expression in HepG2 cells using anti-ATM antibody (Cat#2994, 1:2,000). Green, isotype control; red, ATM.

Immunocytochemical staining of HepG2 cells with anti-ATM antibody (Cat#2994, 1:1,000). Nuclei were stained blue with DAPI; ATM was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-ATM antibody (Cat#2994). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATM antibody (Cat#2994, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

ATM
货号：

2994
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-ATM Rabbit Monoclonal Antibody
Aliases	ATM; ATM Serine/Threonine Kinase; Ataxia Telangiectasia Mutated; TELO1; TEL1; Serine-Protein Kinase ATM; A-T Mutated; EC 2.7.11.1; ATDC; ATA; AT; ATD; Ataxia Telangiectasia Mutated (Includes Complementation Groups A, C And D); TEL1, Telomere Maintenance 1, Homolog (S. Cerevisiae); TEL1, Telomere Maintenance 1, Homolog 3 Serine/Threonine Kinase ATM; AT Mutated; AT1; ATE
Background	Gene Name: ATM NCBI Gene Entry: 472 UniProt Entry: Q13315
Application Information	Molecular Weight: Predicted, 351 kDa; observed, 350 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB3535 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human ATM
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000 Flow Cytometry (FCM): 1:2,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of ATM expression in HepG2 cells using anti-ATM antibody (Cat#2994, 1:2,000). Green, isotype control; red, ATM.
Immunocytochemical staining of HepG2 cells with anti-ATM antibody (Cat#2994, 1:1,000). Nuclei were stained blue with DAPI; ATM was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-ATM antibody (Cat#2994). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATM antibody (Cat#2994, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

ATM
货号：

2994
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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