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Anti-Forkhead Box O3 Rabbit Monoclonal Antibody #2909

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Flow cytometric analysis of Forkhead box O3 expression in HepG2 cells using anti-Forkhead box O3 antibody (Cat#2909, 1:2,000). Green, isotype control; red, Forkhead box O3.

Immunocytochemical staining of HepG2 cells with anti-Forkhead box O3 antibody (Cat#2909, 1:1,000). Nuclei were stained blue with DAPI; Forkhead box O3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

Western blotting analysis using anti-Forkhead box O3 antibody (Cat#2909). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Forkhead box O3 antibody (Cat#2909, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

FOXO3
货号：

2909
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-Forkhead Box O3 Rabbit Monoclonal Antibody
Aliases	FOXO3; Forkhead Box O3; AF6q21; FKHRL; FOXO3A; FOXO; Forkhead In Rhabdomyosarcoma-Like 1; Forkhead Box Protein O; Forkhead, Drosophila, Homolog Of, In Rhabdomyosarcoma-Like 1; Forkhead Homolog (Rhabdomyosarcoma) Like 1; Forkhead Box O3A; AF6q21 Protein; FKHRL1P2; FOXO3A-
Background	Gene Name: FOXO3 NCBI Gene Entry: 2309 UniProt Entry: O43524
Application Information	Molecular Weight: Predicted, 71 kDa; observed, 75 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB3120 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human FoxO3a
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Forkhead box O3 expression in HepG2 cells using anti-Forkhead box O3 antibody (Cat#2909, 1:2,000). Green, isotype control; red, Forkhead box O3.
Immunocytochemical staining of HepG2 cells with anti-Forkhead box O3 antibody (Cat#2909, 1:1,000). Nuclei were stained blue with DAPI; Forkhead box O3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-Forkhead box O3 antibody (Cat#2909). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Forkhead box O3 antibody (Cat#2909, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

FOXO3
货号：

2909
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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