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Anti-EIF4A3 Rabbit Monoclonal Antibody #3009

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Flow cytometric analysis of Eukaryotic translation initiation factor 4A3 expression in HepG2 cells using anti-Eukaryotic translation initiation factor 4A3 antibody (Cat#3009, 1:2,000). Green, isotype control; red, Eukaryotic translation initiation factor 4A3.

Immunocytochemical staining of HepG2 cells with anti-eukaryotic translation initiation factor 4A3 antibody (Cat#3009, 1:1,000). Nuclei were stained blue with DAPI; Eukaryotic translation initiation factor 4A3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

Western blotting analysis using anti-Eukaryotic translation initiation factor 4A3 antibody (Cat#3009). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Eukaryotic translation initiation factor 4A3 antibody (Cat#3009, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

EIF4A3
货号：

3009
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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Information

Product Name	Anti-EIF4A3 Rabbit Monoclonal Antibody
Aliases	EIF4A3; Eukaryotic Translation Initiation Factor 4A3; KIAA0111; EIF4AIII; DDX48; Fal1; Eukaryotic Initiation Factor 4A-Like NUK-34; DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 48; Eukaryotic Initiation Factor 4A-III; ATP-Dependent RNA Helicase EIF4A-3; ATP-Dependent RNA Helicase DDX48; Nuclear Matrix Protein 265; DEAD Box Protein 48; EIF-4A-III; EIF4A-III; NMP 265; Eukaryotic Translation Initiation Factor 4A, Isoform 3; Eukaryotic Translation Initiation Factor 4A Isoform 3; EC 3.6.4.13; HNMP 265; EC 3.6.1; NMP265; MUK34; NUK34; RCPS
Background	Gene Name: EIF4A3 NCBI Gene Entry: 9775 UniProt Entry: P38919
Application Information	Molecular Weight: Predicted, 47 kDa; observed, 47 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB3610 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human eIF4A3
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Eukaryotic translation initiation factor 4A3 expression in HepG2 cells using anti-Eukaryotic translation initiation factor 4A3 antibody (Cat#3009, 1:2,000). Green, isotype control; red, Eukaryotic translation initiation factor 4A3.
Immunocytochemical staining of HepG2 cells with anti-eukaryotic translation initiation factor 4A3 antibody (Cat#3009, 1:1,000). Nuclei were stained blue with DAPI; Eukaryotic translation initiation factor 4A3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-Eukaryotic translation initiation factor 4A3 antibody (Cat#3009). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Eukaryotic translation initiation factor 4A3 antibody (Cat#3009, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

EIF4A3
货号：

3009
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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