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Anti-NONO Rabbit Monoclonal Antibody #3055

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Flow cytometric analysis of NONO expression in HepG2 cells using anti-NONO antibody (Cat#3055, 1:2,000). Green, isotype control; red, NONO.

Immunocytochemical staining of HepG2 cells with anti-NONO antibody (Cat#3055, 1:1,000). Nuclei were stained blue with DAPI; NONO was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

Western blotting analysis using anti-NONO antibody (Cat#3055). Total cell lysates (5 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NONO antibody (Cat#3055, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

NONO
货号：

3055
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-NONO Rabbit Monoclonal Antibody
Aliases	NONO; Non-POU Domain Containing Octamer Binding; NRB54; NMT55; PPP1R114; P54NRB; P54; Non-Pou Domain-Containing Octamer (ATGCAAAT) Binding Protein; Non-POU Domain-Containing Octamer-Binding Protein; Protein Phosphatase 1, Regulatory Subunit 114; DNA-Binding P52/P100 Complex, 52 KDa Subunit; 54 KDa Nuclear RNA- And DNA-Binding Protein; 55 KDa Nuclear Protein; P54(Nrb); Non-POU-Domain-Containing, Octamer-Binding; Nuclear RNA-Binding Protein, 54-KD; NonO Protein; MRXS34; P54nrb
Background	Gene Name: NONO NCBI Gene Entry: 4841 UniProt Entry: Q15233
Application Information	Molecular Weight: Predicted, 54 kDa; observed, 54 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB3840 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human NONO / p54nrb
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of NONO expression in HepG2 cells using anti-NONO antibody (Cat#3055, 1:2,000). Green, isotype control; red, NONO.
Immunocytochemical staining of HepG2 cells with anti-NONO antibody (Cat#3055, 1:1,000). Nuclei were stained blue with DAPI; NONO was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-NONO antibody (Cat#3055). Total cell lysates (5 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NONO antibody (Cat#3055, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

NONO
货号：

3055
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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