Flow cytometric analysis of p62/SQSTM1 expression in HepG2 cells using anti-p62/SQSTM1 antibody (Cat#3086, 1:2,000). Green, isotype control; red, p62/SQSTM1.
Immunocytochemical staining of HepG2 cells with anti-Phospho-FOXO3a (S253) antibody (Cat#3087, 1:1,000) . Nuclei were stained blue with DAPI; Phospho-FOXO3a (S253) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-p62/SQSTM1 antibody (Cat#3086). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-p62/SQSTM1 antibody (Cat#3086, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of p62/SQSTM1 expression in HepG2 cells using anti-p62/SQSTM1 antibody (Cat#3086, 1:2,000). Green, isotype control; red, p62/SQSTM1.
Immunocytochemical staining of HepG2 cells with anti-Phospho-FOXO3a (S253) antibody (Cat#3087, 1:1,000) . Nuclei were stained blue with DAPI; Phospho-FOXO3a (S253) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-p62/SQSTM1 antibody (Cat#3086). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-p62/SQSTM1 antibody (Cat#3086, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of p62/SQSTM1 expression in HepG2 cells using anti-p62/SQSTM1 antibody (Cat#3086, 1:2,000). Green, isotype control; red, p62/SQSTM1.
Immunocytochemical staining of HepG2 cells with anti-Phospho-FOXO3a (S253) antibody (Cat#3087, 1:1,000) . Nuclei were stained blue with DAPI; Phospho-FOXO3a (S253) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-p62/SQSTM1 antibody (Cat#3086). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-p62/SQSTM1 antibody (Cat#3086, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
