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KD-Validated Anti-EIF2AK4 Rabbit Monoclonal Antibody #61956

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Flow cytometric analysis of EIF2AK4 expression in HT-1080 cells using EIF2AK4 antibody (Cat#61956, 1:2,000). Green, isotype control; red, EIF2AK4.
Immunocytochemical staining of HT-1080 cells with anti-EIF2AK4 antibody (Cat #61956, 1:1,000) . Nuclei were stained blue with DAPI; EIF2AK4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-EIF2AK4 antibody (Cat#61956). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-EIF2AK4 antibody (Cat#61956, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-EIF2AK4 antibody (Cat#61956). EIF2AK4 expression in wild type (WT) and EIF2AK4 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-EIF2AK4 antibody (Cat#61956, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    EIF2AK4
  • 货号：
    61956
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-EIF2AK4 Rabbit Monoclonal Antibody
Aliases EIF2AK4; Eukaryotic Translation Initiation Factor 2 Alpha Kinase 4; GCN2; KIAA1338; EIF-2-Alpha Kinase GCN2; GCN2-Like Protein; Eukaryotic Translation Initiation Factor 2-Alpha Kinase 4; General Control Nonderepressible; GCN2 EIF2alpha Kinase; EIF-2-Alpha Kinase; EC 2.7.11.1; PVOD2
Background

Gene Name: EIF2AK4

NCBI Gene Entry: 440275

UniProt Entry: Q9P2K8
Application Information

Molecular Weight: Predicted, 187 kDa, observed, 187 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB5295

Species Reactivity: Human, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human GCN2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of EIF2AK4 expression in HT-1080 cells using EIF2AK4 antibody (Cat#61956, 1:2,000). Green, isotype control; red, EIF2AK4.
  • Immunocytochemical staining of HT-1080 cells with anti-EIF2AK4 antibody (Cat #61956, 1:1,000) . Nuclei were stained blue with DAPI; EIF2AK4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-EIF2AK4 antibody (Cat#61956). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-EIF2AK4 antibody (Cat#61956, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-EIF2AK4 antibody (Cat#61956). EIF2AK4 expression in wild type (WT) and EIF2AK4 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-EIF2AK4 antibody (Cat#61956, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    EIF2AK4
  • 货号：
    61956
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of EIF2AK4 expression in HT-1080 cells using EIF2AK4 antibody (Cat#61956, 1:2,000). Green, isotype control; red, EIF2AK4.
Immunocytochemical staining of HT-1080 cells with anti-EIF2AK4 antibody (Cat #61956, 1:1,000) . Nuclei were stained blue with DAPI; EIF2AK4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-EIF2AK4 antibody (Cat#61956). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-EIF2AK4 antibody (Cat#61956, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-EIF2AK4 antibody (Cat#61956). EIF2AK4 expression in wild type (WT) and EIF2AK4 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-EIF2AK4 antibody (Cat#61956, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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