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KD-Validated Anti-TOP2A Rabbit Polyclonal Antibody #64970

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Western blotting analysis using anti-TOP2A antibody (Cat#64970). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TOP2A antibody (Cat#64970, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis using anti-TOP2A antibody (Cat#64970). TOP2A expression in wild-type (WT) and TOP2A shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-TOP2A antibody (Cat#64970, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

TOP2A
货号：

64970
应用：

WB
物种反应性：

H, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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Information

Product Name	KD-Validated Anti-TOP2A Rabbit Polyclonal Antibody
Aliases	TOP2A; DNA Topoisomerase II Alpha; TOP2alpha; TOPIIA; TOP2; Topoisomerase (DNA) II Alpha 170kDa; DNA Topoisomerase II, Alpha Isozyme; DNA Topoisomerase 2-Alpha; DNA Topoisomerase (ATP-Hydrolyzing); DNA Topoisomerase II, 170 KD; EC 5.99.1.3; DNA Gyrase; EC 5.6.2.2; TP2A
Background	Gene Name: TOP2A NCBI Gene Entry: 7153 UniProt Entry: P11388
Application Information	Molecular Weight: Predicted, 174 kDa; observed, 190 kDa Clonality: Rabbit polyclonal antibody Species Reactivity: Human, rat Applications Tested: Western blotting (WB)
Immunogen	A synthesized peptide derived from human Topoisomerase 2 alpha
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:500-1:2,500
Note	This product is for research use only.

Picture

Western blotting analysis using anti-TOP2A antibody (Cat#64970). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TOP2A antibody (Cat#64970, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-TOP2A antibody (Cat#64970). TOP2A expression in wild-type (WT) and TOP2A shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-TOP2A antibody (Cat#64970, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

TOP2A
货号：

64970
应用：

WB
物种反应性：

H, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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