Anti-Serpin Family H Member 1 Rabbit Monoclonal Antibody - 产品中心

Anti-Serpin Family H Member 1 Rabbit Monoclonal Antibody #3143

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Flow cytometric analysis of Hsp47 expression in H9c2 cells using anti-Hsp47 antibody (Cat#3143, 1:2,000). Green, isotype control; red, Hsp47.

Immunocytochemical staining of H9c2 cells with anti-Hsp47 antibody(Cat #3143, 1:1,000) . Nuclei were stained blue with DAPI; Hsp47 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using anti-Hsp47 antibody (Cat#3143). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Hsp47 antibody (Cat#3143, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

SERPINH1
货号：

3143
应用：

WB, FCM, IC
物种反应性：

M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

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Information

Product Name	Anti-Serpin Family H Member 1 Rabbit Monoclonal Antibody
Aliases	SERPINH1; Serpin Family H Member 1; HSP47; SERPINH2; CBP1; CBP2; Serine (Or Cysteine) Proteinase Inhibitor, Clade H (Heat Shock Protein 47), Member 1, (Collagen Binding Protein 1); Serpin Peptidase Inhibitor, Clade H (Heat Shock Protein 47), Member 1, (Collagen Binding Protein 1); Cell Proliferation-Inducing Gene 14 Protein; Rheumatoid Arthritis-Related Antigen RA-A47; Arsenic-Transactivated Protein 3; Collagen Binding Protein 1; 47 KDa Heat Shock Protein; Heat Shock Protein 47; Serpin H1; Colligin; AsTP3; Serine (Or Cysteine) Proteinase Inhibitor, Clade H (Heat Shock Protein 47), Member 2, (Collagen-Binding Protein 2); Serine (Or Cysteine) Proteinase Inhibitor, Clade H (Heat Shock Protein 47), Member 2; Rheumatoid Arthritis Antigen A-47; Collagen-Binding Protein; Colligin-1; Colligin-2; RA-A47; PIG14; PPROM; OI10; Gp46
Background	Gene Name: SERPINH1 NCBI Gene Entry: 871 UniProt Entry: P50454
Application Information	Molecular Weight: Predicted, 46 kDa; observed, 46 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB5130 Species Reactivity: Mouse, rat Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human Hsp47
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000 Flow Cytometry (FCM) : 1:2,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Hsp47 expression in H9c2 cells using anti-Hsp47 antibody (Cat#3143, 1:2,000). Green, isotype control; red, Hsp47.
Immunocytochemical staining of H9c2 cells with anti-Hsp47 antibody(Cat #3143, 1:1,000) . Nuclei were stained blue with DAPI; Hsp47 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-Hsp47 antibody (Cat#3143). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Hsp47 antibody (Cat#3143, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

SERPINH1
货号：

3143
应用：

WB, FCM, IC
物种反应性：

M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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