Flow cytometric analysis of REEP2 expression in HAP-1 cells using anti-REEP2 antibody (Cat#61889, 1:2,000). Green, isotype control; red, REEP2.
Western blotting analysis using anti-REEP2 antibody (Cat#61889). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-REEP2 antibody (Cat#61889, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-REEP2 antibody (Cat#61889). REEP2 expression in wild-type (WT) and REEP2 knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-REEP2 antibody (Cat#61889, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).