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Anti-Caspase 9 Rabbit Monoclonal Antibody #3153

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Flow cytometric analysis of Caspase 9 expression in C2C12 cells using anti-Caspase 9 antibody (Cat#3153, 1:2,000). Green, isotype control; red, Caspase 9.
Immunocytochemical staining of C2C12 cells with anti-Caspase 9 antibody (Cat#3153, 1:1,000) . Nuclei were stained blue with DAPI; Caspase 9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-caspase 9 antibody (Cat#3153). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-caspase 9 antibody (Cat#3153, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名：
    CASP9
  • 货号：
    3153
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-Caspase 9 Rabbit Monoclonal Antibody
Aliases Caspase 9; ICE-LAP6; APAF-3; MCH6; PPP1R56; Caspase 9, Apoptosis-Related Cysteine Peptidase; Protein Phosphatase 1, Regulatory Subunit 56; ICE-Like Apoptotic Protease 6; Caspase-9; Caspase 9, Apoptosis-Related Cysteine Protease; Apoptotic Protease Activating Factor 3; Apoptotic Protease-Activating Factor 3; Apoptotic Protease MCH-6; Apoptotic Protease Mch-6; EC 3.4.22.62; CASP-9; APAF3
Background

Gene Name: CASP9

NCBI Gene Entry: 842

UniProt Entry: P55211
Application Information

Molecular Weight: Predicted, 50 kDa; observed, 50 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB4270

Species Reactivity: Mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen Recombinant protein of Mouse Caspase-9(UniProt Entry: Q8C3Q9)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Caspase 9 expression in C2C12 cells using anti-Caspase 9 antibody (Cat#3153, 1:2,000). Green, isotype control; red, Caspase 9.
  • Immunocytochemical staining of C2C12 cells with anti-Caspase 9 antibody (Cat#3153, 1:1,000) . Nuclei were stained blue with DAPI; Caspase 9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-caspase 9 antibody (Cat#3153). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-caspase 9 antibody (Cat#3153, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
  • 基因名：
    CASP9
  • 货号：
    3153
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Flow cytometric analysis of Caspase 9 expression in C2C12 cells using anti-Caspase 9 antibody (Cat#3153, 1:2,000). Green, isotype control; red, Caspase 9.
Immunocytochemical staining of C2C12 cells with anti-Caspase 9 antibody (Cat#3153, 1:1,000) . Nuclei were stained blue with DAPI; Caspase 9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-caspase 9 antibody (Cat#3153). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-caspase 9 antibody (Cat#3153, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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