Anti-Crystallin Alpha B Rabbit Monoclonal Antibody - 产品中心

Anti-Crystallin Alpha B Rabbit Monoclonal Antibody #3174

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Flow cytometric analysis of crystallin alpha B expression in H9c2 cells using anti-crystallin alpha B antibody (Cat#3174, 1:2,000). Green, isotype control; red, crystallin alpha B.

Immunocytochemical staining of H9C2 cells with anti-Crystallin alpha B antibody (Cat#3174, 1:1,000) . Nuclei were stained blue with DAPI; Crystallin alpha B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain：Medium. Scale bar, 20 μm.

Western blotting analysis using anti-crystallin alpha B antibody (Cat#3174). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-crystallin alpha B antibody (Cat#3174, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

CRYAB
货号：

3174
应用：

WB, FCM, IC
物种反应性：

R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-Crystallin Alpha B Rabbit Monoclonal Antibody
Aliases	CRYAB; Crystallin Alpha B; HSPB5; CRYA2; Renal Carcinoma Antigen NY-REN-27; Heat Shock Protein Beta-5; Rosenthal Fiber Component; Alpha-Crystallin B Chain; Epididymis Secretory Protein Li 101; Heat-Shock 20 KD Like-Protein; Crystallin, Alpha B; Alpha(B)-Crystallin; HEL-S-101; CTRCT16; CMD1II; CTPP2; HspB5; MFM2
Background	Gene Name: CRYAB NCBI Gene Entry: 1410 UniProt Entry: P02511
Application Information	Molecular Weight: Predicted, 20 kDa; observed, 20 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB4360 Species Reactivity: Rat Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human Alpha B Crystallin
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000 Flow Cytometry (FCM) : 1:2,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of crystallin alpha B expression in H9c2 cells using anti-crystallin alpha B antibody (Cat#3174, 1:2,000). Green, isotype control; red, crystallin alpha B.
Immunocytochemical staining of H9C2 cells with anti-Crystallin alpha B antibody (Cat#3174, 1:1,000) . Nuclei were stained blue with DAPI; Crystallin alpha B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain：Medium. Scale bar, 20 μm.
Western blotting analysis using anti-crystallin alpha B antibody (Cat#3174). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-crystallin alpha B antibody (Cat#3174, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

CRYAB
货号：

3174
应用：

WB, FCM, IC
物种反应性：

R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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