Flow cytometric analysis of CD86 expression in C2C12 cells using anti-CD86 antibody (Cat#3260, 1:2,000). Green, isotype control; red, CD86.
Immunocytochemical staining of C2C12 cells with anti-CD86 antibody (Cat#3260, 1:1,000) . Nuclei were stained blue with DAPI; CD86 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: High. Scale bar, 20 μm.
Immunohistochemistry was performed on paraffin-embedded human tonsillitis using anti-CD86 antibody (Cat#3260, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Western blotting analysis using anti-CD86 antibody (Cat#3260). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD86 antibody (Cat#3260, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
