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Anti-Doublecortin Like Kinase 1 Rabbit Monoclonal Antibody #4709

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Flow cytometric analysis of Doublecortin like kinase 1 expression in C2C12 cells using anti-Doublecortin like kinase 1 antibody ((Cat#4709, 1:2,000). Green, isotype control; red, Doublecortin like kinase 1.
Immunocytochemical staining of C2C12 cells with anti-Doublecortin like kinase 1 antibody ((Cat#4709, 1:1,000) . Nuclei were stained blue with DAPI; Doublecortin like kinase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-doublecortin like kinase 1 antibody (Cat#4709). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-doublecortin like kinase 1 antibody (Cat#4709, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    DCLK1
  • 货号:
    4709
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-Doublecortin Like Kinase 1 Rabbit Monoclonal Antibody
Aliases DCLK1; Doublecortin Like Kinase 1; DCDC3A; KIAA0369; DCAMKL1; DCLK; Doublecortin Domain-Containing Protein 3A; Doublecortin-Like And CAM Kinase-Like 1; Serine/Threonine-Protein Kinase DCLK1; Doublecortin-Like Kinase 1; EC 2.7.11.1; Doublecortin And CaM Kinase-Like 1; EC 2.7.11; CLICK1; CL1
Background

Gene Name: DCLK1

NCBI Gene Entry: 9201

UniProt Entry: O15075

Application Information

Molecular Weight: Predicted, 82 kDa; observed, 75 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB12555

Species Reactivity: Human, mouse

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human DCAMKL1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Doublecortin like kinase 1 expression in C2C12 cells using anti-Doublecortin like kinase 1 antibody ((Cat#4709, 1:2,000). Green, isotype control; red, Doublecortin like kinase 1.
  • Immunocytochemical staining of C2C12 cells with anti-Doublecortin like kinase 1 antibody ((Cat#4709, 1:1,000) . Nuclei were stained blue with DAPI; Doublecortin like kinase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-doublecortin like kinase 1 antibody (Cat#4709). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-doublecortin like kinase 1 antibody (Cat#4709, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Flow cytometric analysis of Doublecortin like kinase 1 expression in C2C12 cells using anti-Doublecortin like kinase 1 antibody ((Cat#4709, 1:2,000). Green, isotype control; red, Doublecortin like kinase 1.
Immunocytochemical staining of C2C12 cells with anti-Doublecortin like kinase 1 antibody ((Cat#4709, 1:1,000) . Nuclei were stained blue with DAPI; Doublecortin like kinase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-doublecortin like kinase 1 antibody (Cat#4709). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-doublecortin like kinase 1 antibody (Cat#4709, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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