Anti-Collagen Type I Alpha 1 Chain Rabbit Monoclonal Antibody - 产品中心

Anti-Collagen Type I Alpha 1 Chain Rabbit Monoclonal Antibody #3265

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Flow cytometric analysis of collagen I alpha 1 expression in H9c2 cells using anti-collagen I alpha 1 antibody (Cat#3265, 1:2,000). Green, isotype control; red, collagen I alpha 1.

Immunocytochemical staining of H9C2 cells with anti-Collagen I alpha 1 antibody (Cat#3265, 1:1,000) . Nuclei were stained blue with DAPI; Collagen I alpha 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain：Medium. Scale bar, 20 μm.

Western blotting analysis using anti-collagen I alpha 1 antibody (Cat#3265). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-collagen I alpha 1 antibody (Cat#3265, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

COL1A1
货号：

3265
应用：

WB, FCM, IC
物种反应性：

M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-Collagen Type I Alpha 1 Chain Rabbit Monoclonal Antibody
Aliases	COL1A1; Collagen Type I Alpha 1 Chain; OI4; Collagen, Type I, Alpha 1; Collagen Alpha-1(I) Chain; Alpha-1 Type I Collagen; Collagen Of Skin, Tendon And Bone,Alpha-1 Chain; Collagen Alpha-1(I) Chain Preproprotein; Type I Procollagen Alpha 1 Chain; Collagen Alpha 1 Chain Type I; Pro-Alpha-1 Collagen Type 1; Alpha1(I) Procollagen; Type I Proalpha 1; EDSARTH1; CAFYD; EDSC; OI1; OI2; OI3
Background	Gene Name: COL1A1 NCBI Gene Entry: 1277 UniProt Entry: P02452
Application Information	Molecular Weight: Predicted, 139 kDa; observed, 220 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB4780 Species Reactivity: Mouse, rat Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)
Immunogen	Recombinant protein of human Collagen I
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000 Flow Cytometry (FCM) : 1:2,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of collagen I alpha 1 expression in H9c2 cells using anti-collagen I alpha 1 antibody (Cat#3265, 1:2,000). Green, isotype control; red, collagen I alpha 1.
Immunocytochemical staining of H9C2 cells with anti-Collagen I alpha 1 antibody (Cat#3265, 1:1,000) . Nuclei were stained blue with DAPI; Collagen I alpha 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain：Medium. Scale bar, 20 μm.
Western blotting analysis using anti-collagen I alpha 1 antibody (Cat#3265). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-collagen I alpha 1 antibody (Cat#3265, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

COL1A1
货号：

3265
应用：

WB, FCM, IC
物种反应性：

M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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