Western blotting analysis using anti-Hexokinase 2 antibody (Cat#52374). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-Hexokinase 2 antibody (Cat#52374, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Human skin was stained with anti-Hexokinase 2 antibody.
Mouse kidney was stained with anti-Hexokinase 2 antibody.
Rat kidney was stained with anti-Hexokinase 2 antibody.
Applications Tested: Western blotting (WB), immunohistochemistry (IHC)
Immunogen
The immunogen information is proprietary.
Isotype
Rabbit IgG,Kappa
Storage Buffer
Supplied in PBS containing 50% glycerol, 0.05% BSA and 0.05% Proclin 300.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000 Immunohistochemistry (IHC): 1:200-1:400
Note
This product is for research use only.
Picture
Western blotting analysis using anti-Hexokinase 2 antibody (Cat#52374). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-Hexokinase 2 antibody (Cat#52374, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Human skin was stained with anti-Hexokinase 2 antibody.
Mouse kidney was stained with anti-Hexokinase 2 antibody.
Rat kidney was stained with anti-Hexokinase 2 antibody.
Western blotting analysis using anti-Hexokinase 2 antibody (Cat#52374). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-Hexokinase 2 antibody (Cat#52374, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Human skin was stained with anti-Hexokinase 2 antibody.
Mouse kidney was stained with anti-Hexokinase 2 antibody.
Rat kidney was stained with anti-Hexokinase 2 antibody.