Anti-NEDD4 E3 Ubiquitin Protein Ligase Rabbit Monoclonal Antibody - 产品中心

Anti-NEDD4 E3 Ubiquitin Protein Ligase Rabbit Monoclonal Antibody #3307

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Flow cytometric analysis of NEDD4 expression in HepG2 cells using anti-NEDD4 antibody (Cat#3307, 1:2,000). Green, isotype control; red, NEDD4.

Immunocytochemical staining of HepG2 cells with anti-NEDD4 antibody(Cat#3307, 1:1,000) . Nuclei were stained blue with DAPI; NEDD4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using anti-NEDD4 antibody (Cat#3307). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NEDD4 antibody (Cat#3307, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

NEDD4
货号：

3307
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-NEDD4 E3 Ubiquitin Protein Ligase Rabbit Monoclonal Antibody
Aliases	NEDD4; NEDD4 E3 Ubiquitin Protein Ligase; NEDD4-1; RPF1; KIAA0093; Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase; Cell Proliferation-Inducing Gene 53 Protein; HECT-Type E3 Ubiquitin Transferase NEDD4; E3 Ubiquitin-Protein Ligase NEDD4; Receptor-Potentiating Factor 1; MGC176705; Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 4; Neural Precursor Cell Expressed, Developmentally Down-Regulated 4; EC 2.3.2.26; EC 6.3.2; NEDD-4
Background	Gene Name: NEDD4 NCBI Gene Entry: 4734 UniProt Entry: P46934
Application Information	Molecular Weight: Predicted, 149 kDa; observed, 130 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB4990 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human NEDD4
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of NEDD4 expression in HepG2 cells using anti-NEDD4 antibody (Cat#3307, 1:2,000). Green, isotype control; red, NEDD4.
Immunocytochemical staining of HepG2 cells with anti-NEDD4 antibody(Cat#3307, 1:1,000) . Nuclei were stained blue with DAPI; NEDD4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-NEDD4 antibody (Cat#3307). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NEDD4 antibody (Cat#3307, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

NEDD4
货号：

3307
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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