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Anti-PDGFRB Rabbit Monoclonal Antibody #3314

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Flow cytometric analysis of PDGFRB expression in C2C12 cells using anti-PDGFRB antibody (Cat#3314, 1:2,000). Green, isotype control; red, PDGFRB.
Immunocytochemical staining of C2C12 cells with anti-PDGFRB antibody (Cat#3314, 1:1,000) . Nuclei were stained blue with DAPI; PDGFRB was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-PDGFRB antibody (Cat#3314). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PDGFRB antibody (Cat#3314, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). PDGFRB, platelet derived growth factor receptor beta.
  • 基因名:
    PDGFRB
  • 货号:
    3314
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-PDGFRB Rabbit Monoclonal Antibody
Aliases PDGFRB; Platelet Derived Growth Factor Receptor Beta; PDGFR1; CD140b; JTK12;
PDGFR; Platelet-Derived Growth Factor Receptor, Beta Polypeptide; Beta-Type Platelet-Derived Growth Factor Receptor; Platelet-Derived Growth Factor Receptor Beta; Platelet-Derived Growth Factor Receptor 1; CD140 Antigen-Like Family Member B; PDGF-R-Beta; EC 2.7.10.1 4; PDGFR-Beta; PDGFR-1; Beta Platelet-Derived Growth Factor Receptor; Activated Tyrosine Kinase PDGFRB; CD140b Antigen; NDEL1-PDGFRB;
EC 2.7.10; IBGC4; PENTT; IMF1; KOGS
Background

Gene Name: PDGFRB

NCBI Gene Entry: 5159

UniProt Entry: P09619

Application Information

Molecular Weight: Predicted, 124 kDa, observed, 190 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB5020

Species Reactivity: Mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human PDGFR beta
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of PDGFRB expression in C2C12 cells using anti-PDGFRB antibody (Cat#3314, 1:2,000). Green, isotype control; red, PDGFRB.
  • Immunocytochemical staining of C2C12 cells with anti-PDGFRB antibody (Cat#3314, 1:1,000) . Nuclei were stained blue with DAPI; PDGFRB was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-PDGFRB antibody (Cat#3314). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PDGFRB antibody (Cat#3314, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). PDGFRB, platelet derived growth factor receptor beta.
Citation(0)
Flow cytometric analysis of PDGFRB expression in C2C12 cells using anti-PDGFRB antibody (Cat#3314, 1:2,000). Green, isotype control; red, PDGFRB.
Immunocytochemical staining of C2C12 cells with anti-PDGFRB antibody (Cat#3314, 1:1,000) . Nuclei were stained blue with DAPI; PDGFRB was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-PDGFRB antibody (Cat#3314). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PDGFRB antibody (Cat#3314, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). PDGFRB, platelet derived growth factor receptor beta.
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