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Anti-EZH2 Mouse Monoclonal Antibody #5386

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Flow cytometric analysis of EZH2 expression in HAP-1 cells using anti-EZH2 antibody (Cat#5386, 1:2,000). Green, isotype control; red, EZH2.

Immunocytochemical staining of HAP-1 cells with anti-EZH2 antibody (Cat#5386, 1:1,000). Nuclei were stained blue with DAPI; EZH2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: High. Scale bar, 20 μm.

Western blotting analysis using anti-EZH2 antibody (Cat#5386). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-EZH2 antibody (Cat#5386, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

EZH2
货号：

5386
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-EZH2 Mouse Monoclonal Antibody
Aliases	EZH2; Enhancer Of Zeste 2 Polycomb Repressive Complex 2 Subunit 2; ENX-1; KMT6; KMT6A; Histone-Lysine N-Methyltransferase EZH2; Lysine N-Methyltransferase 6; Enhancer Of Zeste Homolog 2; EZH1; Enhancer Of Zeste (Drosophila) Homolog 2; Enhancer Of Zeste Homolog 2 (Drosophila); EC 2.1.1.356; EC 2.1.1.43; EC 2.1.1; EZH2b; ENX1; WVS2; WVS
Background	Gene Name: EZH2 NCBI Gene Entry: 2146 UniProt Entry: Q15910
Application Information	Molecular Weight: Predicted, 85 kDa; observed, 98 kDa Clonality: Mouse monoclonal antibody Clone ID: 25GB1260 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	Recombinant protein of human EZH2
Isotype	Mouse IgG1 kappa
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western blotting (WB): 1:500-1:2,500 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of EZH2 expression in HAP-1 cells using anti-EZH2 antibody (Cat#5386, 1:2,000). Green, isotype control; red, EZH2.
Immunocytochemical staining of HAP-1 cells with anti-EZH2 antibody (Cat#5386, 1:1,000). Nuclei were stained blue with DAPI; EZH2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-EZH2 antibody (Cat#5386). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-EZH2 antibody (Cat#5386, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

EZH2
货号：

5386
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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