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Anti-RAPGEF3 Rabbit Monoclonal Antibody #3324

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Flow cytometric analysis of RAPGEF3 expression in C2C12 cells using anti-RAPGEF3 antibody (Cat#3324, 1:2,000). Green, isotype control; red, RAPGEF3.
Immunocytochemical staining of C2C12 cells with anti-RAPGEF3 antibody (Cat#3324, 1:1,000) . Nuclei were stained blue with DAPI; RAPGEF3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：Low. Scale bar, 20 μm.
Western blotting analysis using anti-RAPGEF3 antibody (Cat#3324). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RAPGEF3 antibody (Cat#3324, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). RAPGEF3, rap guanine nucleotide exchange factor 3.
  • 基因名：
    RAPGEF3
  • 货号：
    3324
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-RAPGEF3 Rabbit Monoclonal Antibody
Aliases RAPGEF3; Rap Guanine Nucleotide Exchange Factor 3; CAMP-GEFI; EPAC; Exchange Protein Directly Activated By CAMP 1; Bcm910; Rap1 Guanine-Nucleotide-Exchange Factor Directly Activated By CAMP; CAMP-Regulated Guanine Nucleotide Exchange Factor I; Rap Guanine Nucleotide Exchange Factor (GEF) 3; Exchange Factor Directly Activated By CAMP 1; EPAC 1; EPAC1; RAP Guanine-Nucleotide-Exchange Factor (GEF); 9330170P05Rik; HSU79275; CGEF1
Background

Gene Name: RAPGEF3

NCBI Gene Entry: 10411

UniProt Entry: O95398
Application Information

Molecular Weight: Predicted, 104 kDa; observed, 104 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB5060

Species Reactivity: Mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human Epac1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of RAPGEF3 expression in C2C12 cells using anti-RAPGEF3 antibody (Cat#3324, 1:2,000). Green, isotype control; red, RAPGEF3.
  • Immunocytochemical staining of C2C12 cells with anti-RAPGEF3 antibody (Cat#3324, 1:1,000) . Nuclei were stained blue with DAPI; RAPGEF3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：Low. Scale bar, 20 μm.
  • Western blotting analysis using anti-RAPGEF3 antibody (Cat#3324). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RAPGEF3 antibody (Cat#3324, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). RAPGEF3, rap guanine nucleotide exchange factor 3.
Citation(0)
  • 基因名：
    RAPGEF3
  • 货号：
    3324
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Flow cytometric analysis of RAPGEF3 expression in C2C12 cells using anti-RAPGEF3 antibody (Cat#3324, 1:2,000). Green, isotype control; red, RAPGEF3.
Immunocytochemical staining of C2C12 cells with anti-RAPGEF3 antibody (Cat#3324, 1:1,000) . Nuclei were stained blue with DAPI; RAPGEF3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：Low. Scale bar, 20 μm.
Western blotting analysis using anti-RAPGEF3 antibody (Cat#3324). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RAPGEF3 antibody (Cat#3324, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). RAPGEF3, rap guanine nucleotide exchange factor 3.
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