KD-Validated Anti-RNF31 Mouse Oligoclonal Antibody - 产品中心

KD-Validated Anti-RNF31 Mouse Oligoclonal Antibody #65166

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Flow cytometric analysis of RNF31 expression in HT-1080 cells using anti-RNF31 antibody (Cat#65166, 1:2,000). Green, isotype control; red, RNF31.

Immunocytochemical staining of HT-1080 cells with anti-RNF31 antibody (Cat#65166, 1:1,000). Nuclei were stained blue with DAPI; RNF31 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.

Western blotting analysis using anti-RNF31 antibody (Cat#65166). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RNF31 antibody (Cat#65166, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis using anti-RNF31 antibody (Cat#65166). RNF31 expression in wild-type (WT) and RNF31 knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-RNF31 antibody (Cat#65166, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

RNF31
货号：

65166
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information

Product Name	KD-Validated Anti-RNF31 Mouse Oligoclonal Antibody
Aliases	RNF31; Ring Finger Protein 31; ZIBRA; HOIP; HOIL-1-Interacting Protein; Paul; Zinc In-Between-RING-Finger Ubiquitin-Associated Domain Protein; RING-Type E3 Ubiquitin Transferase RNF31; E3 Ubiquitin-Protein Ligase RNF31; FLJ1011; FLJ23501; RING Finger Protein 31; EC 2.3.2.31; IMD115
Background	Gene Name: RNF31 NCBI Gene Entry: 55072 UniProt Entry: Q96EP0
Application Information	Molecular Weight: Predicted, 120 kDa; observed, 120 kDa Clonality: Mouse oligoclonal antibody Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	Recombinant protein of human RNF31
Isotype	Mouse IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:500-1:2,500 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of RNF31 expression in HT-1080 cells using anti-RNF31 antibody (Cat#65166, 1:2,000). Green, isotype control; red, RNF31.
Immunocytochemical staining of HT-1080 cells with anti-RNF31 antibody (Cat#65166, 1:1,000). Nuclei were stained blue with DAPI; RNF31 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-RNF31 antibody (Cat#65166). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RNF31 antibody (Cat#65166, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-RNF31 antibody (Cat#65166). RNF31 expression in wild-type (WT) and RNF31 knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-RNF31 antibody (Cat#65166, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

RNF31
货号：

65166
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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