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Anti-RAS And EF-Hand Domain Containing Rabbit Monoclonal Antibody #3325

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Flow cytometric analysis of RASEF expression in HepG2 cells using anti-RASEF antibody (Cat#3325, 1:2,000). Green, isotype control; red, RASEF.
Immunocytochemical staining of HepG2 cells with anti-RASEF antibody(Cat#3325, 1:1,000) . Nuclei were stained blue with DAPI; RASEF was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-RASEF antibody (Cat#3325). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RASEF antibody (Cat#3325, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). RASEF, RAS and EF-hand domain containing.
  • 基因名:
    RASEF
  • 货号:
    3325
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-RAS And EF-Hand Domain Containing Rabbit Monoclonal Antibody
Aliases RASEF; RAS And EF-Hand Domain Containing; RAB45; TSG; Ras And EF-Hand Domain-Containing Protein; Ras-Related Protein Rab-45; FLJ31614; RAB45, Member RAS Oncogene Family
Background

Gene Name: RASEF

NCBI Gene Entry: 158158

UniProt Entry: Q8IZ41

Application Information

Molecular Weight: Predicted, 83 kDa; observed, 75 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB5065

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human RAB45
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of RASEF expression in HepG2 cells using anti-RASEF antibody (Cat#3325, 1:2,000). Green, isotype control; red, RASEF.
  • Immunocytochemical staining of HepG2 cells with anti-RASEF antibody(Cat#3325, 1:1,000) . Nuclei were stained blue with DAPI; RASEF was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-RASEF antibody (Cat#3325). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RASEF antibody (Cat#3325, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). RASEF, RAS and EF-hand domain containing.
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Flow cytometric analysis of RASEF expression in HepG2 cells using anti-RASEF antibody (Cat#3325, 1:2,000). Green, isotype control; red, RASEF.
Immunocytochemical staining of HepG2 cells with anti-RASEF antibody(Cat#3325, 1:1,000) . Nuclei were stained blue with DAPI; RASEF was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-RASEF antibody (Cat#3325). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RASEF antibody (Cat#3325, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). RASEF, RAS and EF-hand domain containing.
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