Anti-Ubiquitination Factor E4B Rabbit Monoclonal Antibody - 产品中心

Anti-Ubiquitination Factor E4B Rabbit Monoclonal Antibody #3935

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Flow cytometric analysis of Ubiquitination factor E4 B expression in HepG2 cells using anti-Ubiquitination factor E4 B antibody (Cat#3935, 1:2,000). Green, isotype control; red, Ubiquitination factor E4 B.

Immunocytochemical staining of HepG2 cells with anti-Ubiquitination factor E4 B antibody (Cat#3935, 1:1,000) .Nuclei were stained blue with DAPI; Ubiquitination factor E4 B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-ubiquitination factor E4 B antibody (Cat#3935). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ubiquitination factor E4 B antibody (Cat#3935, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

UBE4B
货号：

3935
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-Ubiquitination Factor E4B Rabbit Monoclonal Antibody
Aliases	UBE4B; Ubiquitination Factor E4B; UFD2; KIAA0684; UBOX3; E4; Ubiquitination Factor E4B (UFD2 Homolog, Yeast); RING-Type E3 Ubiquitin Transferase E4 B; Ubiquitin Conjugation Factor E4 B; HDNB1; Ubiquitination Factor E4B (Homologous To Yeast UFD2); Homozygously Deleted In Neuroblastoma-1; Homozygously Deleted In Neuroblastoma 1; Ubiquitin-Fusion Degradation Protein 2; Ubiquitin Fusion Degradation Protein 2; Homologous To Yeast UFD2; EC 2.3.2.27; UFD2A
Background	Gene Name: UBE4B NCBI Gene Entry: 10277 UniProt Entry: O95155
Application Information	Molecular Weight: Predicted, 146 kDa; observed, 130 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB8765 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human UBE4B
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Ubiquitination factor E4 B expression in HepG2 cells using anti-Ubiquitination factor E4 B antibody (Cat#3935, 1:2,000). Green, isotype control; red, Ubiquitination factor E4 B.
Immunocytochemical staining of HepG2 cells with anti-Ubiquitination factor E4 B antibody (Cat#3935, 1:1,000) .Nuclei were stained blue with DAPI; Ubiquitination factor E4 B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-ubiquitination factor E4 B antibody (Cat#3935). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ubiquitination factor E4 B antibody (Cat#3935, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

UBE4B
货号：

3935
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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