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Anti-Phospho-MCM2 (S108) Rabbit Monoclonal Antibody #3338

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Flow cytometric analysis of phospho-MCM2 (S108) expression in HepG2 cells using anti-phospho-MCM2 (S108) antibody (Cat#3338, 1:2,000). Green, isotype control; red, phospho-MCM2 (S108).
Immunocytochemical staining of HepG2 cells with anti-Phospho-MCM2 (S108) antibody (Cat#3338, 1:1,000) . Nuclei were stained blue with DAPI; Phospho-MCM2 (S108) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-phospho-MCM2 (S108) antibody (Cat#3338). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-MCM2 (S108) antibody (Cat#3338, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    MCM2
  • 货号:
    3338
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-Phospho-MCM2 (S108) Rabbit Monoclonal Antibody
Aliases MCM2; Minichromosome Maintenance Complex Component 2; BM28; KIAA0030; D3S3194; DFNA70; Cdc19; CCNL1; CDCL1; Minichromosome Maintenance Protein 2 Homolog; DNA Replication Licensing Factor MCM2; Nuclear Protein BM28; Mitotin; MCM2 Minichromosome Maintenance Deficient 2, Mitotin (S. Cerevisiae); Minichromosome Maintenance Deficient (S. Cerevisiae) 2 (Mitotin); Minichromosome Maintenance Deficient 2 (Mitotin); Cell Devision Cycle-Like 1; Cyclin-Like 1; EC 3.6.4.12
Background

Gene Name: MCM2

NCBI Gene Entry: 4171

UniProt Entry: P49736

Application Information

Molecular Weight: Predicted, 102 kDa; observed, 125 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB5185

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human Phospho-MCM2 (S108)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Flow Cytometry (FCM): 1:2,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of phospho-MCM2 (S108) expression in HepG2 cells using anti-phospho-MCM2 (S108) antibody (Cat#3338, 1:2,000). Green, isotype control; red, phospho-MCM2 (S108).
  • Immunocytochemical staining of HepG2 cells with anti-Phospho-MCM2 (S108) antibody (Cat#3338, 1:1,000) . Nuclei were stained blue with DAPI; Phospho-MCM2 (S108) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
  • Western blotting analysis using anti-phospho-MCM2 (S108) antibody (Cat#3338). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-MCM2 (S108) antibody (Cat#3338, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Flow cytometric analysis of phospho-MCM2 (S108) expression in HepG2 cells using anti-phospho-MCM2 (S108) antibody (Cat#3338, 1:2,000). Green, isotype control; red, phospho-MCM2 (S108).
Immunocytochemical staining of HepG2 cells with anti-Phospho-MCM2 (S108) antibody (Cat#3338, 1:1,000) . Nuclei were stained blue with DAPI; Phospho-MCM2 (S108) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-phospho-MCM2 (S108) antibody (Cat#3338). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-MCM2 (S108) antibody (Cat#3338, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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