Flow cytometric analysis of phospho-eIF2 alpha (Ser51) expression in HT-1080 cells using anti-phospho-eIF2 alpha (Ser51) antibody (Cat#63068, 1:2,000). Green, isotype control; red, phospho-eIF2 alpha (Ser51).
Immunocytochemical staining of HT-1080 cells with anti-Phospho-eIF2 alpha (Ser51) antibody (Cat#63068, 1:1,000) . Nuclei were stained blue with DAPI; Phospho-eIF2 alpha (Ser51) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-phospho-eIF2 alpha (Ser51) antibody (Cat#63068). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-eIF2 alpha (Ser51) antibody (Cat#63068, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-phospho-eIF2 alpha (Ser51) antibody (Cat#63068). Phospho-eIF2 alpha (Ser51) expression in wild-type (WT) and EIF2S1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-eIF2 alpha (Ser51) antibody (Cat#63068, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). EIF2S1, eukaryotic translation initiation factor 2 subunit alpha.
